Literature DB >> 19081993

Enzyme assays.

Jean-Louis Reymond1, Viviana S Fluxà, Noélie Maillard.   

Abstract

Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign production processes. The present article highlights the aspects of fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting, which are our particular areas of interest.

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Year:  2008        PMID: 19081993     DOI: 10.1039/b813732c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  23 in total

1.  Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes.

Authors:  Orit Redy-Keisar; Einat Kisin-Finfer; Shiran Ferber; Ronit Satchi-Fainaro; Doron Shabat
Journal:  Nat Protoc       Date:  2013-12-05       Impact factor: 13.491

2.  Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases.

Authors:  Alex White; Andrew Koelper; Arielle Russell; Erik M Larsen; Charles Kim; Luke D Lavis; Geoffrey C Hoops; R Jeremy Johnson
Journal:  J Biol Chem       Date:  2018-07-13       Impact factor: 5.157

3.  Imaging lysosomal enzyme activity in live cells using self-quenched substrates.

Authors:  William H Humphries; Christine K Payne
Journal:  Anal Biochem       Date:  2012-03-03       Impact factor: 3.365

Review 4.  Review of methods to probe single cell metabolism and bioenergetics.

Authors:  Andreas E Vasdekis; Gregory Stephanopoulos
Journal:  Metab Eng       Date:  2014-10-31       Impact factor: 9.783

5.  Enzymatic interconversion of isomorphic fluorescent nucleosides: adenosine deaminase transforms an adenosine analogue into an inosine analogue.

Authors:  Renatus W Sinkeldam; Lisa S McCoy; Dongwon Shin; Yitzhak Tor
Journal:  Angew Chem Int Ed Engl       Date:  2013-11-29       Impact factor: 15.336

Review 6.  Miniaturization in biocatalysis.

Authors:  Pedro Fernandes
Journal:  Int J Mol Sci       Date:  2010-03-02       Impact factor: 5.923

7.  Fast, Continuous, and High-Throughput (Bio)Chemical Activity Assay for N-Acyl-l-Homoserine Lactone Quorum-Quenching Enzymes.

Authors:  Daniel Last; Georg H E Krüger; Mark Dörr; Uwe T Bornscheuer
Journal:  Appl Environ Microbiol       Date:  2016-06-30       Impact factor: 4.792

8.  A fluorogenic aldehyde bearing a 1,2,3-triazole moiety for monitoring the progress of aldol reactions.

Authors:  Hai-Ming Guo; Fujie Tanaka
Journal:  J Org Chem       Date:  2009-03-20       Impact factor: 4.354

Review 9.  Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control.

Authors:  Raphaël Billot; Laure Plener; Pauline Jacquet; Mikael Elias; Eric Chabrière; David Daudé
Journal:  J Biol Chem       Date:  2020-07-20       Impact factor: 5.157

Review 10.  Diagnostic testing and interpretation of tests for autoimmunity.

Authors:  Christine Castro; Mark Gourley
Journal:  J Allergy Clin Immunol       Date:  2010-01-12       Impact factor: 10.793

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