Literature DB >> 19079795

Quantum Dots as Reporters in Multiplexed Immunoassays for Biomarkers of Exposure to Agrochemicals.

M Nichkova1, D Dosev, A E Davies, S J Gee, I M Kennedy, B D Hammock.   

Abstract

The application of quantum dots (QDs) as labels in immunoassay microarrays for the multiplex detection of 3-phenoxybenzoic acid (PBA) and atrazine-mercapturate (AM) has been demonstrated. PBA and AM are biomarkers of exposure to the pyrethroid insecticides and to the herbicide atrazine, respectively. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using QDs (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring.

Entities:  

Year:  2007        PMID： 19079795      PMCID： PMC2600528          DOI： 10.1080/00032710701327088

Source DB:  PubMed          Journal:  Anal Lett        ISSN： 0003-2719            Impact factor:   2.329


  25 in total

Review 1.  Patterning proteins and cells using soft lithography.

Authors:  R S Kane; S Takayama; E Ostuni; D E Ingber; G M Whitesides
Journal:  Biomaterials       Date:  1999-12       Impact factor: 12.479

Review 2.  Luminescent quantum dots for multiplexed biological detection and imaging.

Authors:  Warren C W Chan; Dustin J Maxwell; Xiaohu Gao; Robert E Bailey; Mingyong Han; Shuming Nie
Journal:  Curr Opin Biotechnol       Date:  2002-02       Impact factor: 9.740

3.  Pegylated, steptavidin-conjugated quantum dots are effective detection elements for reverse-phase protein microarrays.

Authors:  David Geho; Nicholas Lahar; Prem Gurnani; Michael Huebschman; Paul Herrmann; Virginia Espina; Alice Shi; Julia Wulfkuhle; Harold Garner; Emanuel Petricoin; Lance A Liotta; Kevin P Rosenblatt
Journal:  Bioconjug Chem       Date:  2005 May-Jun       Impact factor: 4.774

4.  Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue.

Authors:  Shuangyang Ding; Junxia Chen; Haiyang Jiang; Jihong He; Weimin Shi; Wenshen Zhao; Jianzhong Shen
Journal:  J Agric Food Chem       Date:  2006-08-23       Impact factor: 5.279

5.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection.

Authors:  W C Chan; S Nie
Journal:  Science       Date:  1998-09-25       Impact factor: 47.728

6.  Semiconductor nanocrystals as fluorescent biological labels.

Authors:  M Bruchez; M Moronne; P Gin; S Weiss; A P Alivisatos
Journal:  Science       Date:  1998-09-25       Impact factor: 47.728

7.  Development of an enzyme-linked immunosorbent assay for atrazine mercapturic acid in human urine.

Authors:  L L Jaeger; A D Jones; B D Hammock
Journal:  Chem Res Toxicol       Date:  1998-04       Impact factor: 3.739

8.  Streptavidin-polyvinylamine conjugates labeled with a europium chelate: applications in immunoassay, immunohistochemistry, and microarrays.

Authors:  A Scorilas; A Bjartell; H Lilja; C Moller; E P Diamandis
Journal:  Clin Chem       Date:  2000-09       Impact factor: 8.327

9.  Room-temperature single-nucleotide polymorphism and multiallele DNA detection using fluorescent nanocrystals and microarrays.

Authors:  Daniele Gerion; Fanqing Chen; Balaji Kannan; Aihua Fu; Wolfgang J Parak; David J Chen; Arunava Majumdar; A Paul Alivisatos
Journal:  Anal Chem       Date:  2003-09-15       Impact factor: 6.986

10.  Improving the signal sensitivity and photostability of DNA hybridizations on microarrays by using dye-doped core-shell silica nanoparticles.

Authors:  Xichun Zhou; Jizhong Zhou
Journal:  Anal Chem       Date:  2004-09-15       Impact factor: 6.986
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  6 in total

1.  Immunoassays and biosensors for monitoring environmental and human exposure to pyrethroid insecticides.

Authors:  Ki Chang Ahn; Hee-Joo Kim; Mark R McCoy; Shirley J Gee; Bruce D Hammock
Journal:  J Agric Food Chem       Date:  2010-11-24       Impact factor: 5.279

2.  Antifouling surface layers for improved signal-to-noise of particle-based immunoassays.

Authors:  Annie Chen; Darby Kozak; Bronwyn J Battersby; Robin M Forrest; Nathalie Scholler; Nicole Urban; Matt Trau
Journal:  Langmuir       Date:  2009-12-01       Impact factor: 3.882

3.  Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid.

Authors:  Can Zhang; Xiaoxiao Wu; Dongyang Li; Jinnuo Hu; Debin Wan; Zhen Zhang; Bruce D Hammock
Journal:  Anal Methods       Date:  2021-03-24       Impact factor: 2.896

Review 4.  Microarray technology for major chemical contaminants analysis in food: current status and prospects.

Authors:  Zhaowei Zhang; Peiwu Li; Xiaofeng Hu; Qi Zhang; Xiaoxia Ding; Wen Zhang
Journal:  Sensors (Basel)       Date:  2012-07-04       Impact factor: 3.576

5.  Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate.

Authors:  Nathan D Israelsen; Donald Wooley; Cynthia Hanson; Elizabeth Vargis
Journal:  J Biol Eng       Date:  2016-01-07       Impact factor: 4.355

Review 6.  Multiplex bioanalytical methods for food and environmental monitoring.

Authors:  Sabina Rebe Raz; Willem Haasnoot
Journal:  Trends Analyt Chem       Date:  2011-07-29       Impact factor: 12.296
  6 in total

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