BACKGROUND/AIMS: We have described VMP1 as a new protein which expression triggers autophagy in mammalian cells. Here we show that experimental diabetes activates VMP1 expression and autophagy in pancreas beta cells as a direct response to streptozotocin (STZ). METHODS: Male Wistar rats were treated with 65 mg/kg STZ and pancreas islets from untreated rats were incubated with 1 mM STZ. RESULTS: RT-PCR analysis shows early VMP1 induction after STZ treatment. In situ hybridization reveals VMP1 mRNA in islet beta cells. Electron microscopy shows chromatin aggregation and autophagy morphology that was confirmed by LC3 expression and LC3-VMP1 co-localization. Apoptotic cell death and the reduction of beta cell pool are evident after 24 h treatment, while VMP1 is still expressed in the remaining cells. VMP1-Beclin1 colocalization in pancreas tissue from STZ-treated rats suggests that VMP1-Beclin1 interaction is involved in the autophagic process activation during experimental diabetes. Results were confirmed using pancreas islets, showing VMP1 expression and autophagy in beta cells as a direct effect of STZ treatment. CONCLUSION: Pancreas beta cells trigger VMP1 expression and autophagy during the early cellular events in response to experimental diabetes. Copyright 2008 S. Karger AG, Basel and IAP.
BACKGROUND/AIMS: We have described VMP1 as a new protein which expression triggers autophagy in mammalian cells. Here we show that experimental diabetes activates VMP1 expression and autophagy in pancreas beta cells as a direct response to streptozotocin (STZ). METHODS: Male Wistar rats were treated with 65 mg/kg STZ and pancreas islets from untreated rats were incubated with 1 mM STZ. RESULTS: RT-PCR analysis shows early VMP1 induction after STZ treatment. In situ hybridization reveals VMP1 mRNA in islet beta cells. Electron microscopy shows chromatin aggregation and autophagy morphology that was confirmed by LC3 expression and LC3-VMP1 co-localization. Apoptotic cell death and the reduction of beta cell pool are evident after 24 h treatment, while VMP1 is still expressed in the remaining cells. VMP1-Beclin1 colocalization in pancreas tissue from STZ-treated rats suggests that VMP1-Beclin1 interaction is involved in the autophagic process activation during experimental diabetes. Results were confirmed using pancreas islets, showing VMP1 expression and autophagy in beta cells as a direct effect of STZ treatment. CONCLUSION: Pancreas beta cells trigger VMP1 expression and autophagy during the early cellular events in response to experimental diabetes. Copyright 2008 S. Karger AG, Basel and IAP.
Authors: Andrea E Lo Ré; Maite G Fernández-Barrena; Luciana L Almada; Lisa D Mills; Sherine F Elsawa; George Lund; Alejandro Ropolo; Maria I Molejon; Maria I Vaccaro; Martin E Fernandez-Zapico Journal: J Biol Chem Date: 2012-04-25 Impact factor: 5.157
Authors: Judit Ribas; Xiaohua Ni; Mark Castanares; Minzhi M Liu; David Esopi; Srinivasan Yegnasubramanian; Ronald Rodriguez; Joshua T Mendell; Shawn E Lupold Journal: Nucleic Acids Res Date: 2012-04-13 Impact factor: 16.971
Authors: Xiaoyong Lei; Robert N Bone; Tomader Ali; Mary Wohltmann; Ying Gai; Karen J Goodwin; Alan E Bohrer; John Turk; Sasanka Ramanadham Journal: Islets Date: 2013-01-01 Impact factor: 2.694