Literature DB >> 19071757

A novel fluorescent method for determination of peroxynitrite using folic acid as a probe.

Jun-Chao Huang1, De-Jia Li, Jun-Chen Diao, Jie Hou, Jiang-Lan Yuan, Guo-Lin Zou.   

Abstract

A novel method for the determination of peroxynitrite using folic acid as a fluorescent probe is described. The method is based on the oxidation of the reduced, low-fluorescent folic acid by peroxynitrite to produce a high-fluorescent emission product. The fluorescence increase is linearly related to the concentration of peroxynitrite in the range of 3x10(-8) to 5.0x10(-6)molL(-1) with a correlation coefficient of 0.998, and the detection limit is 1x10(-8)molL(-1). Interferences from some metal ions normally seen in biological samples, and also some anions structurally similar to peroxynitrite were studied. The optimal conditions for the detection of peroxynitrite were evaluated.

Entities:  

Year:  2007        PMID： 19071757     DOI： 10.1016/j.talanta.2007.01.033

Source DB:  PubMed          Journal:  Talanta        ISSN： 0039-9140            Impact factor:   6.057


  12 in total

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