OBJECTIVE: To investigate the genotoxicity of acrylamide and to determine its genotoxic target organs. METHODS: DNA damage was detected by using comet assay. Lung, liver, spleen, kidney, testicle, bone marrow and peripheral lymphocytes of mice were isolated at 0, 3, 6, 12 and 24h after 50 mg/kg acrylamide intraperitoneal injection. RESULTS: Significant increase in comet tail length, tail DNA% and tail moment in the cells of liver, spleen, testicle, bone marrow and peripheral lymphocytes were induced at different sampling time after treatment of acrylamide. The indexes decreased more or less as time extended. No obvious increase in DNA damages was observed in the lung and kidney with the same treatment. CONCLUSION: Acrylamide could cause DNA damage to multiple organs of mice. The DNA damage caused by acrylamide could be repaired to a certain degree.
OBJECTIVE: To investigate the genotoxicity of acrylamide and to determine its genotoxic target organs. METHODS: DNA damage was detected by using comet assay. Lung, liver, spleen, kidney, testicle, bone marrow and peripheral lymphocytes of mice were isolated at 0, 3, 6, 12 and 24h after 50 mg/kg acrylamide intraperitoneal injection. RESULTS: Significant increase in comet tail length, tail DNA% and tail moment in the cells of liver, spleen, testicle, bone marrow and peripheral lymphocytes were induced at different sampling time after treatment of acrylamide. The indexes decreased more or less as time extended. No obvious increase in DNA damages was observed in the lung and kidney with the same treatment. CONCLUSION:Acrylamide could cause DNA damage to multiple organs of mice. The DNA damage caused by acrylamide could be repaired to a certain degree.