Literature DB >> 190680

Superhelix densities of circular DNA's: a generalized equation for their determination by the bouyant method.

W B Upholt.   

Abstract

Equations for the measurement of the supertwisting of circular DNA's from banding positions in buoyant density gradients containing intercalating dyes have required the use of SV40 DNA isolated from virions as a reference DNA. These equations are modified to allow the use of any closed circular DNA of known superhelix density as a reference DNA.

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Year:  1977        PMID： 190680     DOI： 10.1126/science.190680

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  2 in total

1.  Ethidium bromide-mediated renaturation of denatured closed circular DNAs. The nature of denaturation-resistant fractions of bacteriophage PM2 closed circular DNA.

Authors:  P P Lau; H B Gray
Journal:  Nucleic Acids Res       Date:  1980-02-11       Impact factor: 16.971

2.  Extracellular nucleases of Alteromonas espejiana BAL 31.IV. The single strand-specific deoxyriboendonuclease activity as a probe for regions of altered secondary structure in negatively and positively supercoiled closed circular DNA.

Authors:  P P Lau; H B Gray
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971
  2 in total

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