Literature DB >> 19066943

Shift in polyphenol profile and sublethal phenotype caused by silencing of anthocyanidin synthase in apple (Malus sp.).

Iris Szankowski1, Henryk Flachowsky, Houhua Li, Heidrun Halbwirth, Dieter Treutter, Ionela Regos, Magda-Viola Hanke, Karl Stich, Thilo C Fischer.   

Abstract

We have investigated the consequences of blocking anthocyanin biosynthesis by silencing a key enzyme, anthocyanidin synthase, in transgenic plants of a red-leaved apple cultivar. This is complementary to a previous study of induction of anthocyanin biosynthesis by overexpressing a heterologous transcription factor. Analysis of these opposite phenotypes allows one to study anthocyanin functions in apple and to test the influence of the genetic manipulation on other, related metabolites. As expected, anthocyanin biosynthesis was almost completely blocked and this was accompanied by a shift in the profile of flavonoids and related polyphenols. Most interestingly, a rise in epicatechin was found. A severe reduction of viability by necrotic leaf lesions was also observed, suggesting an essential function of anthocyanins in apple.

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Year:  2008        PMID： 19066943     DOI： 10.1007/s00425-008-0864-4

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  23 in total

1.  Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples.

Authors:  Adam M Takos; Felix W Jaffé; Steele R Jacob; Jochen Bogs; Simon P Robinson; Amanda R Walker
Journal:  Plant Physiol       Date:  2006-09-29       Impact factor: 8.340

2.  Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.

Authors:  Richard Lukacin; Frank Wellmann; Lothar Britsch; Stefan Martens; Ulrich Matern
Journal:  Phytochemistry       Date:  2003-02       Impact factor: 4.072

3.  The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene.

Authors:  A Menssen; S Höhmann; W Martin; P S Schnable; P A Peterson; H Saedler; A Gierl
Journal:  EMBO J       Date:  1990-10       Impact factor: 11.598

Review 4.  MYB transcription factors that colour our fruit.

Authors:  Andrew C Allan; Roger P Hellens; William A Laing
Journal:  Trends Plant Sci       Date:  2008-02-14       Impact factor: 18.313

5.  Developmental changes in the phenol concentrations of 'golden delicious' apple fruits and leaves.

Authors:  U Mayr; D Treutter; C Santos-Buelga; H Bauer; W Feucht
Journal:  Phytochemistry       Date:  1995-03       Impact factor: 4.072

6.  Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation.

Authors:  J. Li; T. M. Ou-Lee; R. Raba; R. G. Amundson; R. L. Last
Journal:  Plant Cell       Date:  1993-02       Impact factor: 11.277

7.  Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin.

Authors:  Yusuke Ban; Chikako Honda; Yoshimichi Hatsuyama; Megumi Igarashi; Hideo Bessho; Takaya Moriguchi
Journal:  Plant Cell Physiol       Date:  2007-05-26       Impact factor: 4.927

8.  Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase.

Authors:  Jonathan J Turnbull; Jun-Ichiro Nakajima; Richard W D Welford; Mami Yamazaki; Kazuki Saito; Christopher J Schofield
Journal:  J Biol Chem       Date:  2003-10-21       Impact factor: 5.157

9.  Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple.

Authors:  David Chagné; Charmaine M Carlisle; Céline Blond; Richard K Volz; Claire J Whitworth; Nnadozie C Oraguzie; Ross N Crowhurst; Andrew C Allan; Richard V Espley; Roger P Hellens; Susan E Gardiner
Journal:  BMC Genomics       Date:  2007-07-03       Impact factor: 3.969

10.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583
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  22 in total

1.  Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa.

Authors:  Houhua Li; Jian Qiu; Fudong Chen; Xiaofen Lv; Chunxiang Fu; Dexiu Zhao; Xuejun Hua; Qiao Zhao
Journal:  Mol Biol Rep       Date:  2011-06-24       Impact factor: 2.316

2.  Color characteristics, pigment accumulation and biosynthetic analyses of leaf color variation in herbaceous peony (Paeonia lactiflora Pall.).

Authors:  Yuhan Tang; Ziwen Fang; Mi Liu; Daqiu Zhao; Jun Tao
Journal:  3 Biotech       Date:  2020-01-28       Impact factor: 2.406

3.  Within-plant distribution of induced resistance in apple seedlings: rapid acropetal and delayed basipetal responses.

Authors:  Bettina Gutbrodt; Karsten Mody; Raphaël Wittwer; Silvia Dorn
Journal:  Planta       Date:  2011-02-15       Impact factor: 4.116

4.  Transgenic apple plants overexpressing the Lc gene of maize show an altered growth habit and increased resistance to apple scab and fire blight.

Authors:  Henryk Flachowsky; Iris Szankowski; Thilo C Fischer; Klaus Richter; Andreas Peil; Monika Höfer; Claudia Dörschel; Sylvia Schmoock; Achim E Gau; Heidrun Halbwirth; Magda-Viola Hanke
Journal:  Planta       Date:  2009-12-05       Impact factor: 4.116

Review 5.  Genetic transformation of fruit trees: current status and remaining challenges.

Authors:  Giorgio Gambino; Ivana Gribaudo
Journal:  Transgenic Res       Date:  2012-03-02       Impact factor: 3.145

6.  QTL and candidate gene mapping for polyphenolic composition in apple fruit.

Authors:  David Chagné; Célia Krieger; Maysoon Rassam; Mike Sullivan; Jenny Fraser; Christelle André; Massimo Pindo; Michela Troggio; Susan E Gardiner; Rebecca A Henry; Andrew C Allan; Tony K McGhie; William A Laing
Journal:  BMC Plant Biol       Date:  2012-01-23       Impact factor: 4.215

7.  Introduction of apple ANR genes into tobacco inhibits expression of both CHI and DFR genes in flowers, leading to loss of anthocyanin.

Authors:  Yuepeng Han; Sornkanok Vimolmangkang; Ruth Elena Soria-Guerra; Schuyler S Korban
Journal:  J Exp Bot       Date:  2012-01-11       Impact factor: 6.992

8.  Fine-tuning of the flavonoid and monolignol pathways during apple early fruit development.

Authors:  Paolo Baldi; Mirko Moser; Matteo Brilli; Urska Vrhovsek; Massimo Pindo; Azeddine Si-Ammour
Journal:  Planta       Date:  2017-02-11       Impact factor: 4.116

9.  RNA-mediated gene silencing signals are not graft transmissible from the rootstock to the scion in greenhouse-grown apple plants Malus sp.

Authors:  Henryk Flachowsky; Conny Tränkner; Iris Szankowski; Sascha Waidmann; Magda-Viola Hanke; Dieter Treutter; Thilo C Fischer
Journal:  Int J Mol Sci       Date:  2012-08-10       Impact factor: 6.208

10.  Transcriptional analysis of apple fruit proanthocyanidin biosynthesis.

Authors:  Rebecca A Henry-Kirk; Tony K McGhie; Christelle M Andre; Roger P Hellens; Andrew C Allan
Journal:  J Exp Bot       Date:  2012-08-01       Impact factor: 6.992
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