OBJECTIVE: To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas. METHOD: A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and UV detection was at 268 nm. RESULT: Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1). CONCLUSION: The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.
OBJECTIVE: To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas. METHOD: A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and UV detection was at 268 nm. RESULT: Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1). CONCLUSION: The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.