Literature DB >> 19065164

Simultaneous widefield single molecule orientation and FRET microscopy in cells.

S E D Webb1, D J Rolfe, S R Needham, S K Roberts, D T Clarke, C I McLachlan, M P Hobson, M L Martin-Fernandez.   

Abstract

We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide data from the epidermal growth factor receptor system in cells. (c) 2008 Optical Society of America

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Year:  2008        PMID： 19065164     DOI： 10.1364/oe.16.020258

Source DB:  PubMed          Journal:  Opt Express        ISSN： 1094-4087            Impact factor:   3.894


  6 in total

1.  Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes.

Authors:  Vanessa DeRocco; Trevor Anderson; Jacob Piehler; Dorothy A Erie; Keith Weninger
Journal:  Biotechniques       Date:  2010-11       Impact factor: 1.993

2.  Automated multidimensional single molecule fluorescence microscopy feature detection and tracking.

Authors:  Daniel J Rolfe; Charles I McLachlan; Michael Hirsch; Sarah R Needham; Christopher J Tynan; Stephen E D Webb; Marisa L Martin-Fernandez; Michael P Hobson
Journal:  Eur Biophys J       Date:  2011-09-18       Impact factor: 1.733

3.  Subcellular and single-molecule imaging of plant fluorescent proteins using total internal reflection fluorescence microscopy (TIRFM).

Authors:  Gema Vizcay-Barrena; Stephen E D Webb; Marisa L Martin-Fernandez; Zoe A Wilson
Journal:  J Exp Bot       Date:  2011-08-23       Impact factor: 6.992

Review 4.  A 'pocket guide' to total internal reflection fluorescence.

Authors:  M L Martin-Fernandez; C J Tynan; S E D Webb
Journal:  J Microsc       Date:  2013-07-24       Impact factor: 1.758

Review 5.  Fluorescence Imaging of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer.

Authors:  Marisa L Martin-Fernandez
Journal:  Cancers (Basel)       Date:  2022-01-28       Impact factor: 6.639

Review 6.  Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives.

Authors:  Marisa L Martin-Fernandez; David T Clarke
Journal:  Int J Mol Sci       Date:  2012-11-13       Impact factor: 5.923
  6 in total

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