| Literature DB >> 19059705 |
Shufang Liang1, Fengming Gong, Xinyu Zhao, Xianhuo Wang, Guobo Shen, Yuhuan Xu, Hanshuo Yang, Xuzhi Ruan, Yuquan Wei.
Abstract
In order to study the characterization of a new tumor-relative FAM92A1-289 protein, we first constructed plasmid FAM92A1-pQE30 for fusion expression in Escherichia coli. The recombinant protein FAM92A1-289 was affinity-purified by Ni2+-charged resin and separated by HPLC chromatography with high purity, and it was further identified by electrospray ionization-mass spectrometry. Furthermore, the expression and cell localization of FAM92A1-289 by immunohistochemistry using our self-prepared polyclonal antibody showed it was expressed in cytoplasm of renal carcinoma. FAM92A1-289 mRNA was expressed in 2 of 10 kidney tissues and in 6 of 12 primary renal tumors. FAM92A1-289 can promote cell growth in vitro and in vivo by colony formation and mouse xenograft assay. Our present data indicated FAM92A1-289 is a new tumor-related gene with oncogenic potentials to probably play roles in renal carcinogenesis.Entities:
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Year: 2008 PMID： 19059705 DOI： 10.1016/j.canlet.2008.10.043
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679