HYPOTHESIS: With the acellular dermal matrix (ADM), it may be possible to bridge large rotator cuff tears and induce tendon regeneration. MATERIALS AND METHODS: A 3 x 5 mm defect of the rotator cuff was created on both shoulders of adult male Sprague-Dawley rats. The graft group (n = 15) underwent reconstruction of the rotator cuff defect with an ADM patch graft; in the defect group (n = 15) no repair was performed. We sacrificed 5 rats from each group at 2, 6, and 12 weeks after surgery and harvested both shoulders; 3 specimens were subjected to histological analysis and the other 7 specimens were used for biomechanical testing. The controls were 5 unoperated rats; they were sacrificed to obtain 3 histologic and 7 biomechanical control shoulder specimens. RESULTS: At each time points, the graft group had significantly higher modified tendon maturing scores than the defect group (p < 0.002); specimens from the graft group demonstrated a greater mean ultimate force to failure than those from the defect group (p < 0.05). Within 12 weeks, the ADM graft was histologically incorporated into a structure resembling control specimen; the mean ultimate force to failure in control was significantly greater than in specimens from both groups (p < 0.01). DISCUSSION: Although the defect was restored in the defect group, histologically and biomechanically specimens from the defect group were inferior to the graft group. CONCLUSION: ADM grafts were useful as a scaffold in the reconstruction of large rotator cuff defects in rats. LEVEL OF EVIDENCE: Basic science study.
HYPOTHESIS: With the acellular dermal matrix (ADM), it may be possible to bridge large rotator cuff tears and induce tendon regeneration. MATERIALS AND METHODS: A 3 x 5 mm defect of the rotator cuff was created on both shoulders of adult male Sprague-Dawley rats. The graft group (n = 15) underwent reconstruction of the rotator cuff defect with an ADM patch graft; in the defect group (n = 15) no repair was performed. We sacrificed 5 rats from each group at 2, 6, and 12 weeks after surgery and harvested both shoulders; 3 specimens were subjected to histological analysis and the other 7 specimens were used for biomechanical testing. The controls were 5 unoperated rats; they were sacrificed to obtain 3 histologic and 7 biomechanical control shoulder specimens. RESULTS: At each time points, the graft group had significantly higher modified tendon maturing scores than the defect group (p < 0.002); specimens from the graft group demonstrated a greater mean ultimate force to failure than those from the defect group (p < 0.05). Within 12 weeks, the ADM graft was histologically incorporated into a structure resembling control specimen; the mean ultimate force to failure in control was significantly greater than in specimens from both groups (p < 0.01). DISCUSSION: Although the defect was restored in the defect group, histologically and biomechanically specimens from the defect group were inferior to the graft group. CONCLUSION: ADM grafts were useful as a scaffold in the reconstruction of large rotator cuff defects in rats. LEVEL OF EVIDENCE: Basic science study.
Authors: Dai Fei Elmer Ker; Dan Wang; Anthony William Behn; Evelyna Tsi Hsin Wang; Xu Zhang; Benjamin Yamin Zhou; Ángel Enrique Mercado-Pagán; Sungwoo Kim; John Kleimeyer; Burhan Gharaibeh; Yaser Shanjani; Drew Nelson; Marc Safran; Emilie Cheung; Phil Campbell; Yunzhi Peter Yang Journal: Adv Funct Mater Date: 2018-03-30 Impact factor: 18.808
Authors: Braden C Fleming; Elise M Magarian; Sophia L Harrison; David J Paller; Martha M Murray Journal: J Orthop Res Date: 2010-06 Impact factor: 3.494
Authors: Tanujan Thangarajah; Catherine J Pendegrass; Shirin Shahbazi; Simon Lambert; Susan Alexander; Gordon W Blunn Journal: Orthop J Sports Med Date: 2015-06-10