BACKGROUND: Obesity is caused by the excessive accumulation of adipose tissue as a result of a chronic energy surplus. Little is known regarding the molecular mechanisms involved in the response to an energy surplus in human adipose tissue at the genomic level. OBJECTIVE: The objective was to investigate changes in the transcriptome of abdominal subcutaneous adipose tissue after a positive energy challenge induced by overfeeding in both lean and obese subjects to identify novel obesity candidate genes. DESIGN: A total of 26 men were recruited and classified on the basis of percentage body fat (measured by dual-energy X-ray absorptiometry) as lean (<20%) or obese (>25%) to participate in the baseline comparison. Sixteen men participated in the overfeeding study (8 lean and 8 obese). Adipose tissue biopsy samples were collected from all subjects at the subumbilical region. Global gene expression profiles were determined at baseline and after a 7-d hypercaloric diet at 40% above normal energy requirements by using whole human genome DNA microarrays. RESULTS: Overfeeding induced differential expression in 45 genes. Six genes displayed a significant interaction effect between adiposity status and overfeeding treatment, including transferrin (TF), stearoyl-CoA desaturase (SCD), transaldolase 1 (TALDO1), cathepsin C (CTSC), insulin receptor substrate 2 (IRS2), and pyruvate dehydrogenase kinase, isozyme 4 (PDK4). Overfeeding resulted in changes in expression of these genes in lean subjects, whereas no significant changes were evident in obese subjects. CONCLUSIONS: Differential expression of these 6 genes may represent a protective mechanism at the molecular level in lean subjects in response to an energy surplus. These genes represent valuable candidates for downstream studies related to obesity.
BACKGROUND:Obesity is caused by the excessive accumulation of adipose tissue as a result of a chronic energy surplus. Little is known regarding the molecular mechanisms involved in the response to an energy surplus in human adipose tissue at the genomic level. OBJECTIVE: The objective was to investigate changes in the transcriptome of abdominal subcutaneous adipose tissue after a positive energy challenge induced by overfeeding in both lean and obese subjects to identify novel obesity candidate genes. DESIGN: A total of 26 men were recruited and classified on the basis of percentage body fat (measured by dual-energy X-ray absorptiometry) as lean (<20%) or obese (>25%) to participate in the baseline comparison. Sixteen men participated in the overfeeding study (8 lean and 8 obese). Adipose tissue biopsy samples were collected from all subjects at the subumbilical region. Global gene expression profiles were determined at baseline and after a 7-d hypercaloric diet at 40% above normal energy requirements by using whole human genome DNA microarrays. RESULTS: Overfeeding induced differential expression in 45 genes. Six genes displayed a significant interaction effect between adiposity status and overfeeding treatment, including transferrin (TF), stearoyl-CoA desaturase (SCD), transaldolase 1 (TALDO1), cathepsin C (CTSC), insulin receptor substrate 2 (IRS2), and pyruvate dehydrogenase kinase, isozyme 4 (PDK4). Overfeeding resulted in changes in expression of these genes in lean subjects, whereas no significant changes were evident in obese subjects. CONCLUSIONS: Differential expression of these 6 genes may represent a protective mechanism at the molecular level in lean subjects in response to an energy surplus. These genes represent valuable candidates for downstream studies related to obesity.
Authors: Linn Gillberg; Alexander Perfilyev; Charlotte Brøns; Martin Thomasen; Louise G Grunnet; Petr Volkov; Fredrik Rosqvist; David Iggman; Ingrid Dahlman; Ulf Risérus; Tina Rönn; Emma Nilsson; Allan Vaag; Charlotte Ling Journal: Diabetologia Date: 2016-01-11 Impact factor: 10.122
Authors: Carmen Hurtado del Pozo; Rosa María Calvo; Gregorio Vesperinas-García; Javier Gómez-Ambrosi; Gema Frühbeck; Miguel Angel Rubio; Maria Jesus Obregon Journal: Obes Surg Date: 2011-05 Impact factor: 4.129
Authors: Xitao Xie; Zhengping Yi; Benjamin Bowen; Cassandra Wolf; Charles R Flynn; Sandeep Sinha; Lawrence J Mandarino; Christian Meyer Journal: J Proteome Res Date: 2010-09-03 Impact factor: 4.466
Authors: Michael Desch; Thomas Schubert; Andrea Schreiber; Sandra Mayer; Björn Friedrich; Ferruh Artunc; Vladimir T Todorov Journal: Mol Endocrinol Date: 2010-09-22
Authors: Jingzhong Ding; Lindsay M Reynolds; Tanja Zeller; Christian Müller; Kurt Lohman; Barbara J Nicklas; Stephen B Kritchevsky; Zhiqing Huang; Alberto de la Fuente; Nicola Soranzo; Robert E Settlage; Chia-Chi Chuang; Timothy Howard; Ning Xu; Mark O Goodarzi; Y-D Ida Chen; Jerome I Rotter; David S Siscovick; John S Parks; Susan Murphy; David R Jacobs; Wendy Post; Russell P Tracy; Philipp S Wild; Stefan Blankenberg; Ina Hoeschele; David Herrington; Charles E McCall; Yongmei Liu Journal: Diabetes Date: 2015-07-07 Impact factor: 9.461