| Literature DB >> 1905137 |
H Sztajer1, J Borkowski, K Sobiech.
Abstract
Lipase (triacylglycerol lipase, EC 3.1.1.3) has been purified from Pseudomonas fluorescens wild strain by chromatography on DEAE-cellulose and octyl-Sepharose CL-4B. The yield was 21% and the specific activity of the purified enzyme 4780 U/mg protein. It showed a Mr of about 45 x 10(4) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme is active over a wide pH range and at 50-55 degrees C.Entities:
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Year: 1991 PMID： 1905137
Source DB: PubMed Journal: Biotechnol Appl Biochem ISSN： 0885-4513 Impact factor: 2.431