Literature DB >> 1904842

Purification and characterization of an extracellular 29-kilodalton phospholipase C from Listeria monocytogenes.

C Geoffroy1, J Raveneau, J L Beretti, A Lecroisey, J A Vazquez-Boland, J E Alouf, P Berche.   

Abstract

We purified and characterized an extracellular phospholipase produced by Listeria monocytogenes. This enzyme was separated as a homogeneous protein of 29 kDa by chromatography on DEAE-52 cellulose and Bio-Gel P100 columns. It is a zinc-dependent phospholipase C (PLC) that is mainly active at pH 6 to 7 and expresses lecithinase activity and a weaker sphingomyelinase activity. The exoenzyme also hydrolyzed phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin but not phosphatidylinositol. It was distinct from the 36-kDa phosphatidylinositol PLC produced by L. monocytogenes and from the L. ivanovii sphingomyelinase. The pure protein expressed a weak, calcium-independent hemolytic activity and was not toxic in mice. Western immunoblot analysis using a rabbit immune serum raised against the enzyme showed that all virulent strains of L. monocytogenes tested produced in the culture supernatant a 29-kDa PLC. In contrast, no proteins antigenically related to the 29-kDa PLC were detected in supernatants of L. ivanovii, L. seeligeri, L. innocua, or L. welshimeri. The role in virulence of the 29-kDa PLC specifically produced by L. monocytogenes remains to be established.

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Year:  1991        PMID： 1904842      PMCID： PMC258022          DOI： 10.1128/iai.59.7.2382-2388.1991

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  39 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Cell-mediated immunity to bacterial infection in the mouse. Thymus-derived cells as effectors of acquired resistance to Listeria monocytogenes.

Authors:  R V Blanden; R E Langman
Journal:  Scand J Immunol       Date:  1972       Impact factor: 3.487

Review 3.  Clostridium perfringens toxins (type A, B, C, D, E).

Authors:  J L McDonel
Journal:  Pharmacol Ther       Date:  1980       Impact factor: 12.310

4.  Purification and characterization of hemolysin produced by Listeria monocytogenes.

Authors:  I H Siddique; I F Lin; R A Chung
Journal:  Am J Vet Res       Date:  1974-02       Impact factor: 1.156

5.  Listeria monocytogenes-haemolysin: lecithinase.

Authors:  M A Khan; T A Seaman; M Woodbine
Journal:  Zentralbl Bakteriol Orig A       Date:  1973-10

6.  Production of phospholipase C (alpha-toxin), haemolysins and lethal toxins by Clostridium perfringens types A to D.

Authors:  R Möllby; T Holme; C E Nord; C J Smyth; T Wadström
Journal:  J Gen Microbiol       Date:  1976-09

7.  The metal ion dependence of phospholipase C from Bacillus cereus.

Authors:  C Little; A B Otnåss
Journal:  Biochim Biophys Acta       Date:  1975-06-24

8.  Extracellular antigens from Listeria monocytogenes. II. Cytotoxicity of hemolytic and lipolytic antigens of Listeria for cultured mouse macrophages.

Authors:  B B Watson; J C Lavizzo
Journal:  Infect Immun       Date:  1973-05       Impact factor: 3.441

9.  Cellular mediators of anti-Listeria immunity as an enlarged population of short lived, replicating T cells. Kinetics of their production.

Authors:  R J North
Journal:  J Exp Med       Date:  1973-08-01       Impact factor: 14.307

10.  Requirement of thymus (T) lymphocytes for resistance to listeriosis.

Authors:  F C Lane; E R Unanue
Journal:  J Exp Med       Date:  1972-05-01       Impact factor: 14.307
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  56 in total

1.  pH-regulated activation and release of a bacteria-associated phospholipase C during intracellular infection by Listeria monocytogenes.

Authors:  H Marquis; E J Hager
Journal:  Mol Microbiol       Date:  2000-01       Impact factor: 3.501

Review 2.  Molecular determinants of Listeria monocytogenes pathogenesis.

Authors:  D A Portnoy; T Chakraborty; W Goebel; P Cossart
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441

3.  The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction.

Authors:  A Bubert; S Köhler; W Goebel
Journal:  Appl Environ Microbiol       Date:  1992-08       Impact factor: 4.792

4.  Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF-kappaB (RelA/p50) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IkappaBalpha and IkappaBbeta degradation.

Authors:  N Hauf; W Goebel; F Fiedler; Z Sokolovic; M Kuhn
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-19       Impact factor: 11.205

Review 5.  Listeria pathogenesis and molecular virulence determinants.

Authors:  J A Vázquez-Boland; M Kuhn; P Berche; T Chakraborty; G Domínguez-Bernal; W Goebel; B González-Zorn; J Wehland; J Kreft
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

6.  The virulence gene cluster of Listeria monocytogenes is also present in Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species.

Authors:  E Gouin; J Mengaud; P Cossart
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

7.  The two distinct phospholipases C of Listeria monocytogenes have overlapping roles in escape from a vacuole and cell-to-cell spread.

Authors:  G A Smith; H Marquis; S Jones; N C Johnston; D A Portnoy; H Goldfine
Journal:  Infect Immun       Date:  1995-11       Impact factor: 3.441

8.  Membrane permeabilization by Listeria monocytogenes phosphatidylinositol-specific phospholipase C is independent of phospholipid hydrolysis and cooperative with listeriolysin O.

Authors:  H Goldfine; C Knob; D Alford; J Bentz
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

9.  Phosphatidylcholine-specific phospholipase C from Listeria monocytogenes is an important virulence factor in murine cerebral listeriosis.

Authors:  D Schlüter; E Domann; C Buck; T Hain; H Hof; T Chakraborty; M Deckert-Schlüter
Journal:  Infect Immun       Date:  1998-12       Impact factor: 3.441

10.  The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi.

Authors:  Philipp Aurass; Maren Schlegel; Omar Metwally; Clare R Harding; Gunnar N Schroeder; Gad Frankel; Antje Flieger
Journal:  J Biol Chem       Date:  2013-03-01       Impact factor: 5.157
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