Literature DB >> 19047394

Cytomorphological and genetic characterization of troglobitic Leptolyngbya strains isolated from Roman hypogea.

Laura Bruno1, Daniela Billi, Simona Bellezza, Patrizia Albertano.   

Abstract

Six Leptolyngbya strains, isolated from the archaeological surfaces of hypogean sites, were phenotypically and genetically characterized by light and electron microscopy and 16S rRNA gene and 16S-23S internally transcribed spacer (ITS) sequencing. Three phycoerythrin-rich (red) and three phycocyanin-rich (green) isolates were assigned to different operational taxonomic units (OTUs). Among the green isolates, one strain showed an OTU intraspecific variation due to differences in the ITS sequences and genomic polymorphism. Within the ITS sequence, variable regions, conserved domains and tRNA(Ile) and tRNA(Ala) genes showed high sequence identity among the phylotypes. Together, these data indicated a relatedness of the six strains to other Leptolyngbya from subaerophytic and geothermal environments and allowed the definition of novel Leptolyngbya OTUs.

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Year:  2008        PMID: 19047394      PMCID: PMC2632135          DOI: 10.1128/AEM.01183-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  26 in total

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