OBJECTIVE: To investigate the effect of beta-carotene against testicular injury induced by methotrexate (MTX). DESIGN: Experimental study. SETTING: Animal and histology laboratory at Inonu University. ANIMAL(S): Twenty-eight Wistar male rats. INTERVENTION(S): Twenty-eight rats were separated into four groups: control, beta-carotene, MTX, and beta-carotene + MTX. At the end of the treatment, the animals were killed, and tissue samples were examined via histologic and biochemical methods. MAIN OUTCOME MEASURE(S): In each group, 100 tubules were classified as intact, sloughing, atrophic, and degenerated. Caspase-3, a universal effector of apoptosis, was evaluated according to staining in place of coloring as weak, mild, and strong. RESULT(S): In the MTX group, 58.5 + 3.7% of tubules were sloughing, 10.8 + 2.1% of tubules were atrophic, and 2.0 + 0.6% of tubules were degenerative. In the beta-carotene + MTX group, the affected tubule number was statistically significantly lower than in the MTX group. The distribution of caspase-3 in the MTX group showed a statistically significant increase, but it decreased in the beta-carotene + MTX group. The enzyme activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GP-x) and the level of malondialdehyde (MDA) increased and decreased in parallel. CONCLUSION(S): Our results indicate that beta-carotene may be useful in decreasing the side effects of chemotherapy, including apoptotic cell death.
OBJECTIVE: To investigate the effect of beta-carotene against testicular injury induced by methotrexate (MTX). DESIGN: Experimental study. SETTING: Animal and histology laboratory at Inonu University. ANIMAL(S): Twenty-eight Wistar male rats. INTERVENTION(S): Twenty-eight rats were separated into four groups: control, beta-carotene, MTX, and beta-carotene + MTX. At the end of the treatment, the animals were killed, and tissue samples were examined via histologic and biochemical methods. MAIN OUTCOME MEASURE(S): In each group, 100 tubules were classified as intact, sloughing, atrophic, and degenerated. Caspase-3, a universal effector of apoptosis, was evaluated according to staining in place of coloring as weak, mild, and strong. RESULT(S): In the MTX group, 58.5 + 3.7% of tubules were sloughing, 10.8 + 2.1% of tubules were atrophic, and 2.0 + 0.6% of tubules were degenerative. In the beta-carotene + MTX group, the affected tubule number was statistically significantly lower than in the MTX group. The distribution of caspase-3 in the MTX group showed a statistically significant increase, but it decreased in the beta-carotene + MTX group. The enzyme activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GP-x) and the level of malondialdehyde (MDA) increased and decreased in parallel. CONCLUSION(S): Our results indicate that beta-carotene may be useful in decreasing the side effects of chemotherapy, including apoptotic cell death.
Authors: Mansur Daggulli; Onur Dede; Mehmet Mazhar Utangac; Mehmet Nuri Bodakci; Namık Kemal Hatipoglu; Necmettin Penbegul; Ahmet Ali Sancaktutar; Yaşar Bozkurt; Gül Türkçü; Hatice Yüksel Journal: Int J Clin Exp Med Date: 2014-12-15
Authors: Igor Sukhotnik; Omri Nativ; Alex Roitburt; Daniel Bejar; Arnold G Coran; Jorge G Mogilner; Ofer Nativ Journal: Pediatr Surg Int Date: 2013-02 Impact factor: 1.827
Authors: Chunmei Lin; Yun Seok Choi; Seul Gi Park; Lee Wha Gwon; Jong Geol Lee; Jung-Min Yon; In-Jeoung Baek; Beom Jun Lee; Young Won Yun; Sang-Yoon Nam Journal: Biomed Res Int Date: 2016-12-05 Impact factor: 3.411