Literature DB >> 19041907

An enzyme cocktail for efficient protoplast formation in Aspergillus niger.

Charissa de Bekker1, Ad Wiebenga, Guillermo Aguilar, Han A B Wösten.   

Abstract

Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia.

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Year:  2008        PMID： 19041907     DOI： 10.1016/j.mimet.2008.11.001

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  17 in total

1.  Chitinase production in solid-state fermentation from Oerskovia xanthineolytica NCIM 2839 and its application in fungal protoplasts formation.

Authors:  Shailesh R Waghmare; Swaroop S Kulkarni; Jai S Ghosh
Journal:  Curr Microbiol       Date:  2011-07-10       Impact factor: 2.188

2.  Overexpression, purification and characterisation of homologous α-L-arabinofuranosidase and endo-1,4-β-D-glucanase in Aspergillus vadensis.

Authors:  Helena Culleton; Vincent A McKie; Ronald P de Vries
Journal:  J Ind Microbiol Biotechnol       Date:  2014-09-27       Impact factor: 3.346

3.  FlbA-Regulated Gene rpnR Is Involved in Stress Resistance and Impacts Protein Secretion when Aspergillus niger Is Grown on Xylose.

Authors:  David Aerts; Stijn G van den Bergh; Harm Post; Maarten A F Altelaar; Mark Arentshorst; Arthur F J Ram; Robin A Ohm; Han A B Wösten
Journal:  Appl Environ Microbiol       Date:  2019-01-09       Impact factor: 4.792

4.  Breeding and identification of novel koji molds with high activity of acid protease by genome recombination between Aspergillus oryzae and Aspergillus niger.

Authors:  Defeng Xu; Li Pan; Haifeng Zhao; Mouming Zhao; Jiaxin Sun; Dongmei Liu
Journal:  J Ind Microbiol Biotechnol       Date:  2010-11-24       Impact factor: 3.346

5.  Single cell transcriptomics of neighboring hyphae of Aspergillus niger.

Authors:  Charissa de Bekker; Oskar Bruning; Martijs J Jonker; Timo M Breit; Han A B Wösten
Journal:  Genome Biol       Date:  2011-08-04       Impact factor: 13.583

6.  Heterologous Synthesis of Monacolin J by Reconstructing Its Biosynthetic Gene Cluster in Aspergillus niger.

Authors:  Xu Zeng; Junwei Zheng; Feifei Lu; Li Pan; Bin Wang
Journal:  J Fungi (Basel)       Date:  2022-04-16

7.  Penicillium ochrochloron MTCC 517 chitinase: An effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi.

Authors:  Nilambari S Patil; Jyoti P Jadhav
Journal:  Saudi J Biol Sci       Date:  2014-10-05       Impact factor: 4.219

8.  Intergeneric fusant development using chitinase preparation of Rhizopus stolonifer NCIM 880.

Authors:  Kailas D Sonawane; Narayan R Dandagal; Akibjaved G Naikwadi; Piyush T Gurav; Samar V Anapat; Naiem H Nadaf; Deepak B Jadhav; Shailesh R Waghmare
Journal:  AMB Express       Date:  2016-11-14       Impact factor: 3.298

9.  Construction of Aspergillus niger integrated with cellulase gene from Ampullaria gigas Spix for improved enzyme production and saccharification of alkaline-pretreated rice straw.

Authors:  Peizhou Yang; Haifeng Zhang; Lili Cao; Zhi Zheng; Shaotong Jiang
Journal:  3 Biotech       Date:  2016-11-04       Impact factor: 2.406

10.  Cytosolic streaming in vegetative mycelium and aerial structures of Aspergillus niger.

Authors:  R Bleichrodt; A Vinck; P Krijgsheld; M R van Leeuwen; J Dijksterhuis; H A B Wösten
Journal:  Stud Mycol       Date:  2012-09-14       Impact factor: 16.097
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