Literature DB >> 1904125

Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b.

D L Cardy1, V Laidler, G P Salmond, J C Murrell.   

Abstract

The oxidation of methane to methanol in methanotrophic bacteria is catalysed by the enzyme methane monooxygenase (MM0). This multicomponent enzyme catalyses a range of oxidations including that of aliphatic and aromatic compounds and therefore has potential for commercial exploitation. This study details the molecular characterization of the soluble MMO (sMMO) genes from the Type II methanotroph Methylosinus trichosporium OB3b. The structural genes encoding the alpha, beta and gamma subunits of sMMO protein A and the structural gene encoding component B have been isolated and sequenced. These genes have been expressed and their products identified using an in vitro system. A comparative analysis of sMMO predicted sequences of M. trichosporium OB3b and the taxonomically related M. capsulatus (Bath) is also presented.

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Year:  1991        PMID： 1904125     DOI： 10.1111/j.1365-2958.1991.tb02114.x

Source DB:  PubMed          Journal:  Mol Microbiol        ISSN： 0950-382X            Impact factor:   3.501


  36 in total

1.  Detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoA, mmoX, mxaF, and 16S rRNA and ribosomal DNA, including pmoA-based terminal restriction fragment length polymorphism profiling.

Authors:  H P Horz; M T Yimga; W Liesack
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

2.  Saturation mutagenesis of toluene ortho-monooxygenase of Burkholderia cepacia G4 for Enhanced 1-naphthol synthesis and chloroform degradation.

Authors:  Lingyun Rui; Young Man Kwon; Ayelet Fishman; Kenneth F Reardon; Thomas K Wood
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

3.  Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor.

Authors:  J L Caballero; E Martinez; F Malpartida; D A Hopwood
Journal:  Mol Gen Genet       Date:  1991-12

Review 4.  Molecular ecology techniques for the study of aerobic methanotrophs.

Authors:  Ian R McDonald; Levente Bodrossy; Yin Chen; J Colin Murrell
Journal:  Appl Environ Microbiol       Date:  2007-12-28       Impact factor: 4.792

5.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

6.  Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation.

Authors:  Keith A Canada; Sachiyo Iwashita; Hojae Shim; Thomas K Wood
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

7.  Soluble Methane Monooxygenase Production and Trichloroethylene Degradation by a Type I Methanotroph, Methylomonas methanica 68-1.

Authors:  S C Koh; J P Bowman; G S Sayler
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792

8.  Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge.

Authors:  K Watanabe; M Teramoto; H Futamata; S Harayama
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

9.  Detection of methanotrophs in groundwater by PCR.

Authors:  Y S Cheng; J L Halsey; K A Fode; C C Remsen; M L Collins
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792

10.  The methane monooxygenase gene cluster of Methylosinus trichosporium: cloning and sequencing of the mmoC gene.

Authors:  D L Cardy; V Laidler; G P Salmond; J C Murrell
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552
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