OBJECTIVE: To investigate the possible participation of angiotensin II receptor 1 (AT(1) receptor) in pressor response induced by IL-1beta in the paraventricular nucleus (PVN) of hypothalamus. METHODS: The experiments were performed on adult Wistar rats. Mean arterial pressure (MAP) were recorded during microinjection of different doses of IL-1beta, IL-1beta together with Losartan, an AT1 receptor antagonist, or Ang II pre-treated with IL-1beta into PVN. Rats microinjected with saline were served as controls in all experiments. RESULTS: Microinjection of IL-1beta (0.1 or 0.2 ng) resulted in increases in blood pressure in a dose-dependent manner, and the pressor effect caused by administration of IL-1beta (0.2 ng) could be attenuated by pre-treatment with AT(1) receptor antagonist (100 ng Losartan). A subpressor dose of IL-1beta (0.05 ng) could augment the pressor response induced by microinjection of Ang II (10 ng). DISCUSSION: The present results provide support that central infusion of IL-1beta increases blood pressure and the pressor response requires stimulation of AT(1) receptors. It is also shown that IL-1beta has the effect of increasing the magnitude of pressor response induced by Ang II in PVN.
OBJECTIVE: To investigate the possible participation of angiotensin II receptor 1 (AT(1) receptor) in pressor response induced by IL-1beta in the paraventricular nucleus (PVN) of hypothalamus. METHODS: The experiments were performed on adult Wistar rats. Mean arterial pressure (MAP) were recorded during microinjection of different doses of IL-1beta, IL-1beta together with Losartan, an AT1 receptor antagonist, or Ang II pre-treated with IL-1beta into PVN. Rats microinjected with saline were served as controls in all experiments. RESULTS: Microinjection of IL-1beta (0.1 or 0.2 ng) resulted in increases in blood pressure in a dose-dependent manner, and the pressor effect caused by administration of IL-1beta (0.2 ng) could be attenuated by pre-treatment with AT(1) receptor antagonist (100 ng Losartan). A subpressor dose of IL-1beta (0.05 ng) could augment the pressor response induced by microinjection of Ang II (10 ng). DISCUSSION: The present results provide support that central infusion of IL-1beta increases blood pressure and the pressor response requires stimulation of AT(1) receptors. It is also shown that IL-1beta has the effect of increasing the magnitude of pressor response induced by Ang II in PVN.
Authors: Jiandong Zhang; Nathan P Rudemiller; Mehul B Patel; Norah S Karlovich; Min Wu; Alicia A McDonough; Robert Griffiths; Matthew A Sparks; Alexander D Jeffs; Steven D Crowley Journal: Cell Metab Date: 2015-12-17 Impact factor: 27.287