OBJECTIVE: The Flavivirus genera share epitopes inducing cross-reactive antibodies leading to great difficulty in differentially diagnosing flaviviral infections. This work was aimed at evaluating the complexity of dengue and yellow fever serological differential diagnosis. MATERIAL AND METHODS: Dengue antibody capture ELISA and a yellow fever neutralisation test were carried out on 13 serum samples obtained from yellow fever patients, 20 acute serum samples from dengue patients and 19 voluntary serum samples pre- and post-vaccination with YF vaccine. RESULTS: Dengue ELISA revealed IgM reactivity in 46,2 % of yellow fever patients and 42 % of vaccinees. Sixteen out of 20 dengue patients (80 %) had high YF virus neutralisation titres. CONCLUSIONS: Such very high cross-reactivity data challenged differential laboratory diagnosis of dengue and yellow fever in areas where both flaviviruses co-circulate. New laboratory strategies are thus needed for improving the tests and providing a specific laboratory diagnosis. Cross-reactivity between Flaviviruses represents a great difficulty for epidemiological surveillance and preventing dengue, both of which demand urgent attention.
OBJECTIVE: The Flavivirus genera share epitopes inducing cross-reactive antibodies leading to great difficulty in differentially diagnosing flaviviral infections. This work was aimed at evaluating the complexity of dengue and yellow fever serological differential diagnosis. MATERIAL AND METHODS: Dengue antibody capture ELISA and a yellow fever neutralisation test were carried out on 13 serum samples obtained from yellow feverpatients, 20 acute serum samples from dengue patients and 19 voluntary serum samples pre- and post-vaccination with YF vaccine. RESULTS: Dengue ELISA revealed IgM reactivity in 46,2 % of yellow feverpatients and 42 % of vaccinees. Sixteen out of 20 dengue patients (80 %) had high YF virus neutralisation titres. CONCLUSIONS: Such very high cross-reactivity data challenged differential laboratory diagnosis of dengue and yellow fever in areas where both flaviviruses co-circulate. New laboratory strategies are thus needed for improving the tests and providing a specific laboratory diagnosis. Cross-reactivity between Flaviviruses represents a great difficulty for epidemiological surveillance and preventing dengue, both of which demand urgent attention.
Authors: David A Lindholm; Todd Myers; Susana Widjaja; Edward M Grant; Kalyani Telu; Tahaniyat Lalani; Jamie Fraser; Mary Fairchok; Anuradha Ganesan; Mark D Johnson; Anjali Kunz; David R Tribble; Heather C Yun Journal: Am J Trop Med Hyg Date: 2017-01-23 Impact factor: 2.345
Authors: Andréa Barbosa de Melo; Maria da Paz C da Silva; Maria Cecília F Magalhães; Laura Helena Vega Gonzales Gil; Eduardo M Freese de Carvalho; Ulisses M Braga-Neto; Giovani Rota Bertani; Ernesto T A Marques; Marli Tenório Cordeiro Journal: Am J Trop Med Hyg Date: 2011-10 Impact factor: 2.345
Authors: Oliver J Brady; Peter W Gething; Samir Bhatt; Jane P Messina; John S Brownstein; Anne G Hoen; Catherine L Moyes; Andrew W Farlow; Thomas W Scott; Simon I Hay Journal: PLoS Negl Trop Dis Date: 2012-08-07
Authors: Natalie B Cleton; Gert-Jan Godeke; Johan Reimerink; Mathias F Beersma; H Rogier van Doorn; Leticia Franco; Marco Goeijenbier; Miguel A Jimenez-Clavero; Barbara W Johnson; Matthias Niedrig; Anna Papa; Vittorio Sambri; Adriana Tami; Zoraida I Velasco-Salas; Marion P G Koopmans; Chantal B E M Reusken Journal: PLoS Negl Trop Dis Date: 2015-03-13