| Literature DB >> 19038510 |
Isabel Fidalgo-Carvalho1, Jodi K Craigo, Shannon Barnes, Carolina Costa-Ramos, Ronald C Montelaro.
Abstract
EIAV is a monocyte/macrophage tropic virus. To date, even though EIAV has been under investigation for numerous years, very few details have been elucidated about EIAV/macrophage interactions. This is largely due to the absence of an equine macrophage cell line that would support viral replication. Herein we describe the spontaneous immortalization and generation of a clonal equine macrophage-like (EML) cell line with the functional and immunophenotype characteristics of differentiated equine monocyte derived macrophage(s) (eMDM(s)). These cells possess strong non-specific esterase (NSE) activity, are able to phagocytose fluorescent bioparticles, and produce nitrites in response to LPS. The EML-3C cell line expresses the EIAV receptor for cellular entry (ELR1) and supports replication of the virulent EIAV(PV) biological clone. Thus, EML-3C cells provide a useful cell line possessing equine macrophage related properties for the growth and study of EIAV infection as well as of other equine macrophage tropic viruses.Entities:
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Year: 2008 PMID： 19038510 PMCID： PMC2689946 DOI： 10.1016/j.vetmic.2008.10.010
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293