BACKGROUND: We have explored a gene-therapeutic approach to stimulate antitumor immunity by adenoviral-mediated transfer of CD40 ligand (CD40L) to treat metastatic liver cancer in a rat model. MATERIALS AND METHODS: Rat metastatic liver cancer cells were implanted into the back of rats bilaterally. When the larger tumor reached 8.0 mm in diameter, adenovirus vector-expressing mouse CD40L was injected intratumorally as treatment group (n=5), while LacZ was injected in the control group (n=5). RESULTS: In the control group, the tumor gradually grew to be 20.7+/-1.6 (mean+/-SD) mm in intratumorally injected tumors and 21.8+/-3.7 mm in opposite tumors seven weeks after injection, respectively. In contrast, in the treatment group, the tumor was reduced to 3.6+/-8.2 mm and 3.7+/-8.2 mm. The tumor growth and survival rate were significantly different (p<0.001). CONCLUSION: Adenovirus vector-mediated CD40L gene therapy is an effective therapeutic method for metastatic liver cancer.
BACKGROUND: We have explored a gene-therapeutic approach to stimulate antitumor immunity by adenoviral-mediated transfer of CD40 ligand (CD40L) to treat metastatic liver cancer in a rat model. MATERIALS AND METHODS:Rat metastatic liver cancer cells were implanted into the back of rats bilaterally. When the larger tumor reached 8.0 mm in diameter, adenovirus vector-expressing mouseCD40L was injected intratumorally as treatment group (n=5), while LacZ was injected in the control group (n=5). RESULTS: In the control group, the tumor gradually grew to be 20.7+/-1.6 (mean+/-SD) mm in intratumorally injected tumors and 21.8+/-3.7 mm in opposite tumors seven weeks after injection, respectively. In contrast, in the treatment group, the tumor was reduced to 3.6+/-8.2 mm and 3.7+/-8.2 mm. The tumor growth and survival rate were significantly different (p<0.001). CONCLUSION: Adenovirus vector-mediated CD40L gene therapy is an effective therapeutic method for metastatic liver cancer.
Authors: Elizabeth A Thompson; Frank Liang; Gustaf Lindgren; Kerrie J Sandgren; Kylie M Quinn; Patricia A Darrah; Richard A Koup; Robert A Seder; Ross M Kedl; Karin Loré Journal: J Immunol Date: 2015-06-29 Impact factor: 5.422
Authors: Shaymaa M M Yahya; Salma M Abdelnasser; Ahmad R Hamed; Osama H El Sayed; Mohsen S Asker Journal: Mol Biol Rep Date: 2019-09-06 Impact factor: 2.316