Ying Wu1, Meng-Tao Li, Yan Zhao, Sheng Feng, Li Su, Yi Dong. 1. Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Abstract
OBJECTIVE: To detect anti-M3 receptor antibodies in primary Sjögren's syndrome (pSS) patients and to explore its association with clinical manifestations. METHODS: Anti-M3 antibodies were tested in 70 patients with pSS , 50 patients with systemic lupus erythematosus (SLE) and 76 normal controls with ELISA and Western blot. The correlation between anti-M3 antibodies and other clinical manifestations was analyzed. RESULTS: (1) The positive rate of anti-M3 antibodies in pSS was 47.14% using ELISA and 60.00% using Western blot in SLE 4.00% using ELISA and 12.00% using Western blot and in normal controls 14.47% using ELISA and 15.79% using Western blot. (2) The incidence of schirmer test, tear break-up time (BUT), whole saliva flow rate, punctate epithelial erosions (PEE) on the corneas and external eye examination were not significantly different between the anti-M3 positive and negative groups. (3) The incidences of IgG, rheumatoid factor, SSB and fluorescence index (FI) > 3 were higher in the positive group than in the negative group using ELISA. CONCLUSION: The positive rate of anti-M3 antibodies is higher in pSS than in SLE and normal controls and in some degree it has correlation with the lesion in salivary gland.
OBJECTIVE: To detect anti-M3 receptor antibodies in primary Sjögren's syndrome (pSS) patients and to explore its association with clinical manifestations. METHODS: Anti-M3 antibodies were tested in 70 patients with pSS , 50 patients with systemic lupus erythematosus (SLE) and 76 normal controls with ELISA and Western blot. The correlation between anti-M3 antibodies and other clinical manifestations was analyzed. RESULTS: (1) The positive rate of anti-M3 antibodies in pSS was 47.14% using ELISA and 60.00% using Western blot in SLE 4.00% using ELISA and 12.00% using Western blot and in normal controls 14.47% using ELISA and 15.79% using Western blot. (2) The incidence of schirmer test, tear break-up time (BUT), whole saliva flow rate, punctate epithelial erosions (PEE) on the corneas and external eye examination were not significantly different between the anti-M3 positive and negative groups. (3) The incidences of IgG, rheumatoid factor, SSB and fluorescence index (FI) > 3 were higher in the positive group than in the negative group using ELISA. CONCLUSION: The positive rate of anti-M3 antibodies is higher in pSS than in SLE and normal controls and in some degree it has correlation with the lesion in salivary gland.
Authors: María Pía Negroni; Gabriel L Fiszman; María E Azar; Carlos Cresta Morgado; Alejandro J Español; Laura T Pelegrina; Eulalia de la Torre; María Elena Sales Journal: J Clin Immunol Date: 2010-02-16 Impact factor: 8.317