AIM: To determine the expression of DNA (MMR) proteins, including hMLH1 and hMSH2, in gastric epithelial cells in the patients with or without Helicobacter pylori (H pylori)-infected gastritis. METHODS: Fifty H pylori-positive patients and 50 H pylori-negative patients were enrolled in the study. During endoscopy of patients with non-ulcer dyspepsia, two antral and two corpus biopsies were taken for histological examination (Giemsa stain) and for immunohistochemical staining of hMLH1 and hMSH2. RESULTS: The percentage of epithelial cell nuclei that demonstrated positivity for hMLH1 staining was 84.14 +/- 7.32% in H pylori-negative patients, while it was 73.34 +/- 10.10% in H pylori-positive patients (P < 0.0001). No significant difference was seen between the two groups regarding the percentage of epithelial cell nuclei that demonstrated positivity for hMSH2 staining (81.16 +/- 8.32% in H pylori-negative versus 78.24 +/- 8.71% in H pylori-positive patients; P = 0.09). CONCLUSION: This study indicates that H pylori might promote development of gastric carcinoma at least in part through its ability to affect the DNA MMR system.
AIM: To determine the expression of DNA (MMR) proteins, including hMLH1 and hMSH2, in gastric epithelial cells in the patients with or without Helicobacter pylori (H pylori)-infected gastritis. METHODS: Fifty H pylori-positive patients and 50 H pylori-negative patients were enrolled in the study. During endoscopy of patients with non-ulcer dyspepsia, two antral and two corpus biopsies were taken for histological examination (Giemsa stain) and for immunohistochemical staining of hMLH1 and hMSH2. RESULTS: The percentage of epithelial cell nuclei that demonstrated positivity for hMLH1 staining was 84.14 +/- 7.32% in H pylori-negative patients, while it was 73.34 +/- 10.10% in H pylori-positive patients (P < 0.0001). No significant difference was seen between the two groups regarding the percentage of epithelial cell nuclei that demonstrated positivity for hMSH2 staining (81.16 +/- 8.32% in H pylori-negative versus 78.24 +/- 8.71% in H pylori-positive patients; P = 0.09). CONCLUSION: This study indicates that H pylori might promote development of gastric carcinoma at least in part through its ability to affect the DNA MMR system.
Authors: Jae J Kim; Hong Tao; Emilia Carloni; Wai K Leung; David Y Graham; Antonia R Sepulveda Journal: Gastroenterology Date: 2002-08 Impact factor: 22.682
Authors: Dawit Kidane; Wook Jin Chae; Jennifer Czochor; Kristin A Eckert; Peter M Glazer; Alfred L M Bothwell; Joann B Sweasy Journal: Crit Rev Biochem Mol Biol Date: 2014-01-13 Impact factor: 8.250
Authors: Woo Chul Chung; Sung Hoon Jung; Kang Moon Lee; Chang Nyol Paik; Jae Wuk Kwak; Ji Han Jung; Jin Young Yoo; Min Kyoung Lee; In-Sik Chung Journal: Gut Liver Date: 2010-06-16 Impact factor: 4.519