Zuzana Kunická1, Igor Mucha, Jirí Fajkus. 1. Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, CZ-62500 Brno, Czech Republic.
Abstract
BACKGROUND: Telomerase activity is associated with many malignancies, including head and neck cancer. The use of telomerase activity as a diagnostic and prognostic marker of head and neck cancer development was examined and compared with standard histological analysis. PATIENTS AND METHODS: Telomerase activity was determined using quantitative dual-colour real-time TRAP (telomeric repeat amplification protocol). In each of 58 patients, a sample of tumour tissue, adjacent mucosa and normal muscle was collected. RESULTS: Telomerase activation was observed in 88% of tumour tissues and 34% of tumour-adjacent mucosa samples. No telomerase activity was detected in normal muscle tissues. Telomerase activity correlated with tumour grade, with an average of 4.6 telomerase units (T.U.) in well-differentiated, 8.3 T.U. in moderately-differentiated and 20 T.U. in poorly differentiated tumours. Relapse occurred in 13 patients and no telomerase activity was detected in 3 recurrent tumours. CONCLUSION: Telomerase activity may be used as an objective parameter inversely related to tumour differentiation. Prognosis in telomerase-negative tumours is worse than that of the telomerase-positive group.
BACKGROUND: Telomerase activity is associated with many malignancies, including head and neck cancer. The use of telomerase activity as a diagnostic and prognostic marker of head and neck cancer development was examined and compared with standard histological analysis. PATIENTS AND METHODS: Telomerase activity was determined using quantitative dual-colour real-time TRAP (telomeric repeat amplification protocol). In each of 58 patients, a sample of tumour tissue, adjacent mucosa and normal muscle was collected. RESULTS: Telomerase activation was observed in 88% of tumour tissues and 34% of tumour-adjacent mucosa samples. No telomerase activity was detected in normal muscle tissues. Telomerase activity correlated with tumour grade, with an average of 4.6 telomerase units (T.U.) in well-differentiated, 8.3 T.U. in moderately-differentiated and 20 T.U. in poorly differentiated tumours. Relapse occurred in 13 patients and no telomerase activity was detected in 3 recurrent tumours. CONCLUSION: Telomerase activity may be used as an objective parameter inversely related to tumour differentiation. Prognosis in telomerase-negative tumours is worse than that of the telomerase-positive group.
Authors: Michal Zalzman; W Alex Meltzer; Benjamin A Portney; Robert A Brown; Aditi Gupta Journal: Curr Issues Mol Biol Date: 2019-08-18 Impact factor: 2.081
Authors: Paolo Boscolo-Rizzo; Maria Cristina Da Mosto; Enrica Rampazzo; Silvia Giunco; Annarosa Del Mistro; Anna Menegaldo; Lorena Baboci; Monica Mantovani; Giancarlo Tirelli; Anita De Rossi Journal: Cancer Metastasis Rev Date: 2016-09 Impact factor: 9.264