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Literature DB >> 1902954

Purification of merozoites of Theileria sergenti from infected bovine erythrocytes.

C Sugimoto¹, M Sato, S Kawazu, T Kamio, K Fujisaki.

Abstract

A simple and rapid method for purifying merozoites of Theileria sergenti from infected bovine erythrocytes was developed. Infected erythrocytes were lysed by the use of a cytolytic toxin produced by Aeromonas hydrophila and the lysate was subjected to ultracentrifugation in a Percoll discontinuous density gradient. Pure and morphologically intact merozoites free of erythrocyte membrane were recovered from a band formed at the interface of 40% and 60% (by vol.) Percoll solutions. In this merozoite fraction, contamination of erythrocyte membrane proteins was not detected as examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Entities: Chemical Species

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Year: 1991 PMID： 1902954 DOI： 10.1007/bf00935426

Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289

10 in total

1. Antigenic properties of Theileria sergenti in ELISA serodiagnosis.

Authors: T Ohgitani; T Okabe; N Sasaki
Journal: Nihon Juigaku Zasshi Date: 1987-06

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. The use of aerolysin toxin as an aid for visualization of low numbers of African trypanosomes in whole blood.

Authors: T W Pearson; L E Saya; S P Howard; J T Buckley
Journal: Acta Trop Date: 1982-03 Impact factor: 3.112

4. Isolation of Theileria parva schizonts from infected lymphoblastoid cells.

Authors: C Sugimoto; P A Conrad; S Ito; W C Brown; D J Grab
Journal: Acta Trop Date: 1988-09 Impact factor: 3.112

5. Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins.

Authors: J J Edwards; N G Anderson; S L Nance; N L Anderson
Journal: Blood Date: 1979-06 Impact factor: 22.113

6. Purification and some properties of Aeromonas hydrophila hemolysin.

Authors: T Asao; Y Kinoshita; S Kozaki; T Uemura; G Sakaguchi
Journal: Infect Immun Date: 1984-10 Impact factor: 3.441

7. Purification and some properties of the hemolytic toxin aerolysin.

Authors: J T Buckley; L N Halasa; K D Lund; S MacIntyre
Journal: Can J Biochem Date: 1981-06

8. Theileria orientalis, a cosmopolitan blood parasite of cattle: demonstration of the schizont stage.

Authors: G Uilenberg; N M Perié; A A Spanjer; F F Franssen
Journal: Res Vet Sci Date: 1985-05 Impact factor: 2.534

9. Isolation of Theileria sergenti piroplasms from infected erythrocytes and development of an enzyme-linked immunosorbent assay for serodiagnosis of T sergenti infections.

Authors: S Shimizu; K Suzuki; K Nakamura; K Kadota; K Fujisaki; S Ito; T Minami
Journal: Res Vet Sci Date: 1988-09 Impact factor: 2.534

10. The presence of Theileria sergenti in Haemaphysalis longicornis overwintering in pasture in Japan.

Authors: K Fujisaki; Y Ito; T Kamio; S Kitaoka
Journal: Ann Trop Med Parasitol Date: 1985-10

10 in total

6 in total

1. Antigenic differences between Japanese Theileria sergenti and other benign Theileria species of cattle from Australia (T. buffeli) and Britain (T. orientalis).

Authors: S Kawazu; C Sugimoto; T Kamio; K Fujisaki
Journal: Parasitol Res Date: 1992 Impact factor: 2.289

2. Detection of Theileria sergenti infection in cattle by polymerase chain reaction amplification of parasite-specific DNA.

Authors: M Tanaka; S Onoe; T Matsuba; S Katayama; M Yamanaka; H Yonemichi; K Hiramatsu; B K Baek; C Sugimoto; M Onuma
Journal: J Clin Microbiol Date: 1993-10 Impact factor: 5.948

3. Characterization and comparison of merozoite antigens of different Babesia canis isolates by serological and immunological investigations.

Authors: S Hauschild; P Shayan; E Schein
Journal: Parasitol Res Date: 1995 Impact factor: 2.289