OBJECTIVES: To measure the expression levels of B-lymphocyte stimulator (BLyS) and its receptors in multiple myeloma (MM), and to investigate the relationship between them. DESIGN AND METHODS: BLyS and its receptors mRNA levels from peripheral blood mononuclear cells (PBMCs) of 31 MM patients and 30 healthy controls were determined by real-time PCR. The results were presented as the ratios of target genes, to beta(2)-microglobulin (beta(2)M), mRNA. BLyS serum level was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The circulating levels of BLyS in 31 MM patients were significantly elevated and correlated with the levels of Lactate dehydrogenase (LDH). More importantly, 93.55% (29/31) and 90.32% (28/31) of these MM patients had significantly higher expression levels of BLyS and BCMA mRNA respectively, which are correlated with the levels of LDH, while 64.52% (20/31) of these MM patients had significantly lower expression levels of TACI mRNA. CONCLUSION: BLyS protein concentration and BLyS, TACI and BAFF-R mRNA expression levels were significantly elevated in patients with MM, suggesting that BLyS, TACI and BAFF-R may be involved in the pathogenesis of MM, and that expression levels could serve as a biomarker of prognosis.
OBJECTIVES: To measure the expression levels of B-lymphocyte stimulator (BLyS) and its receptors in multiple myeloma (MM), and to investigate the relationship between them. DESIGN AND METHODS: BLyS and its receptors mRNA levels from peripheral blood mononuclear cells (PBMCs) of 31 MMpatients and 30 healthy controls were determined by real-time PCR. The results were presented as the ratios of target genes, to beta(2)-microglobulin (beta(2)M), mRNA. BLyS serum level was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The circulating levels of BLyS in 31 MMpatients were significantly elevated and correlated with the levels of Lactate dehydrogenase (LDH). More importantly, 93.55% (29/31) and 90.32% (28/31) of these MMpatients had significantly higher expression levels of BLyS and BCMA mRNA respectively, which are correlated with the levels of LDH, while 64.52% (20/31) of these MMpatients had significantly lower expression levels of TACI mRNA. CONCLUSION:BLyS protein concentration and BLyS, TACI and BAFF-R mRNA expression levels were significantly elevated in patients with MM, suggesting that BLyS, TACI and BAFF-R may be involved in the pathogenesis of MM, and that expression levels could serve as a biomarker of prognosis.
Authors: Marilena Kampa; George Notas; Efstathios N Stathopoulos; Andreas Tsapis; Elias Castanas Journal: Front Oncol Date: 2020-06-16 Impact factor: 6.244
Authors: Molly Went; Amit Sud; Asta Försti; Britt-Marie Halvarsson; Niels Weinhold; Scott Kimber; Mark van Duin; Gudmar Thorleifsson; Amy Holroyd; David C Johnson; Ni Li; Giulia Orlando; Philip J Law; Mina Ali; Bowang Chen; Jonathan S Mitchell; Daniel F Gudbjartsson; Rowan Kuiper; Owen W Stephens; Uta Bertsch; Peter Broderick; Chiara Campo; Obul R Bandapalli; Hermann Einsele; Walter A Gregory; Urban Gullberg; Jens Hillengass; Per Hoffmann; Graham H Jackson; Karl-Heinz Jöckel; Ellinor Johnsson; Sigurður Y Kristinsson; Ulf-Henrik Mellqvist; Hareth Nahi; Douglas Easton; Paul Pharoah; Alison Dunning; Julian Peto; Federico Canzian; Anthony Swerdlow; Rosalind A Eeles; ZSofia Kote-Jarai; Kenneth Muir; Nora Pashayan; Jolanta Nickel; Markus M Nöthen; Thorunn Rafnar; Fiona M Ross; Miguel Inacio da Silva Filho; Hauke Thomsen; Ingemar Turesson; Annette Vangsted; Niels Frost Andersen; Anders Waage; Brian A Walker; Anna-Karin Wihlborg; Annemiek Broyl; Faith E Davies; Unnur Thorsteinsdottir; Christian Langer; Markus Hansson; Hartmut Goldschmidt; Martin Kaiser; Pieter Sonneveld; Kari Stefansson; Gareth J Morgan; Kari Hemminki; Björn Nilsson; Richard S Houlston Journal: Nat Commun Date: 2018-09-13 Impact factor: 14.919
Authors: De-Xiu Bu; Reshma Singh; Eugene E Choi; Marco Ruella; Selene Nunez-Cruz; Keith G Mansfield; Paul Bennett; Nathanial Barton; Qilong Wu; Jiquan Zhang; Yongqiang Wang; Lai Wei; Shawn Cogan; Tucker Ezell; Shree Joshi; Kellie J Latimer; Brian Granda; William R Tschantz; Regina M Young; Heather A Huet; Celeste J Richardson; Michael C Milone Journal: Oncotarget Date: 2018-05-25