Literature DB >> 1902800

Purification of two thermostable components of messenger ribonucleoprotein particles (mRNPs) from Xenopus laevis oocytes, belonging to a novel class of RNA-binding proteins.

S Deschamps1, A Viel, H Denis, M le Maire.   

Abstract

We have purified and partially sequenced two proteins from Xenopus laevis previtellogenic oocytes, belonging to messenger ribonucleoprotein particles (mRNPs). The purification procedure rests on the thermostability of these proteins, which remain soluble after heating the cell extracts at 80 degrees C. The thermostable proteins can be identified with two of the most abundant components (mRNP3 and mRNP4) of the mRNPs, described by Darnbrough and Ford (1981) [Eur. J. Biochem. 118, 415-424]. mRNP3 and mRNP4 are homologous to each other, but to no other protein of known sequence. The abundance and semi-periodic distribution of proline residues in mRNP3 and mRNP4 sequences suggest that these RNA-binding proteins adopt an unusual type of conformation.

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Year:  1991        PMID： 1902800     DOI： 10.1016/0014-5793(91)80456-d

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes.

Authors:  K Matsumoto; K Aoki; N Dohmae; K Takio; M Tsujimoto
Journal:  Nucleic Acids Res       Date:  2000-12-01       Impact factor: 16.971

2.  Binding of Xenopus oocyte masking proteins to mRNA sequences.

Authors:  K Marello; J LaRovere; J Sommerville
Journal:  Nucleic Acids Res       Date:  1992-11-11       Impact factor: 16.971

3.  Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.

Authors:  M T Murray; D L Schiller; W W Franke
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

Review 4.  Transcription and masking of mRNA in germ cells: involvement of Y-box proteins.

Authors:  J Sommerville; M Ladomery
Journal:  Chromosoma       Date:  1996-04       Impact factor: 4.316

5.  Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes.

Authors:  M Ladomery; E Wade; J Sommerville
Journal:  Nucleic Acids Res       Date:  1997-03-01       Impact factor: 16.971

6.  Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway.

Authors:  M Braddock; M Muckenthaler; M R White; A M Thorburn; J Sommerville; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971

7.  Promoter control of translation in Xenopus oocytes.

Authors:  N Gunkel; M Braddock; A M Thorburn; M Muckenthaler; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1995-02-11       Impact factor: 16.971

8.  Binding of Y-box proteins to RNA: involvement of different protein domains.

Authors:  M Ladomery; J Sommerville
Journal:  Nucleic Acids Res       Date:  1994-12-25       Impact factor: 16.971

9.  Two forms of elongation factor 1 alpha (EF-1 alpha O and 42Sp50), present in oocytes, but absent in somatic cells of Xenopus laevis.

Authors:  S Deschamps; J Morales; A Mazabraud; M le Maire; H Denis; D D Brown
Journal:  J Cell Biol       Date:  1991-09       Impact factor: 10.539
  9 in total

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