Literature DB >> 19027798

A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum.

Matthew W A Dixon1, Christopher L Peatey, Donald L Gardiner, Katharine R Trenholme.   

Abstract

Study of the formation of the sexual blood stages of the malaria parasite has been significantly hampered by the absence of a reliable, reproducible assay devoid of operator bias and error. Here we report on the development of an assay utilizing a green fluorescent protein chimera of the early sexual blood stage protein Pfs16 as a marker for commitment to gametocytogenesis. Analysis of parasites via fluorescence activated cell sorting allows for the accurate assessment of gametocyte production well before morphological changes are apparent.

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Year:  2008        PMID： 19027798     DOI： 10.1016/j.molbiopara.2008.10.004

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  22 in total

1.  Quantitative assessment of Plasmodium falciparum sexual development reveals potent transmission-blocking activity by methylene blue.

Authors:  Sophie H Adjalley; Geoffrey L Johnston; Tao Li; Richard T Eastman; Eric H Ekland; Abraham G Eappen; Adam Richman; B Kim Lee Sim; Marcus C S Lee; Stephen L Hoffman; David A Fidock
Journal:  Proc Natl Acad Sci U S A       Date:  2011-10-31       Impact factor: 11.205

Review 2.  Sexual development in Plasmodium parasites: knowing when it's time to commit.

Authors:  Gabrielle A Josling; Manuel Llinás
Journal:  Nat Rev Microbiol       Date:  2015-09       Impact factor: 60.633

3.  Antimalarial asexual stage-specific and gametocytocidal activities of HIV protease inhibitors.

Authors:  Christopher L Peatey; Katherine T Andrews; Nina Eickel; Timothy MacDonald; Alice S Butterworth; Katharine R Trenholme; Donald L Gardiner; James S McCarthy; Tina S Skinner-Adams
Journal:  Antimicrob Agents Chemother       Date:  2009-12-22       Impact factor: 5.191

4.  Soft X-ray microscopy analysis of cell volume and hemoglobin content in erythrocytes infected with asexual and sexual stages of Plasmodium falciparum.

Authors:  Eric Hanssen; Christian Knoechel; Megan Dearnley; Matthew W A Dixon; Mark Le Gros; Carolyn Larabell; Leann Tilley
Journal:  J Struct Biol       Date:  2011-09-16       Impact factor: 2.867

5.  The spiroindolone drug candidate NITD609 potently inhibits gametocytogenesis and blocks Plasmodium falciparum transmission to anopheles mosquito vector.

Authors:  J C van Pelt-Koops; H E Pett; W Graumans; M van der Vegte-Bolmer; G J van Gemert; M Rottmann; B K S Yeung; T T Diagana; R W Sauerwein
Journal:  Antimicrob Agents Chemother       Date:  2012-04-16       Impact factor: 5.191

6.  Production of Plasmodium falciparum gametocytes in vitro.

Authors:  Kathryn Shaw Saliba; Marcelo Jacobs-Lorena
Journal:  Methods Mol Biol       Date:  2013

7.  Flow cytometry for the evaluation of anti-plasmodial activity of drugs on Plasmodium falciparum gametocytes.

Authors:  Séverine Chevalley; Agnès Coste; Alexandrine Lopez; Bernard Pipy; Alexis Valentin
Journal:  Malar J       Date:  2010-02-11       Impact factor: 2.979

8.  Lysine acetylation in sexual stage malaria parasites is a target for antimalarial small molecules.

Authors:  Katharine Trenholme; Linda Marek; Sandra Duffy; Gabriele Pradel; Gillian Fisher; Finn K Hansen; Tina S Skinner-Adams; Alice Butterworth; Che Julius Ngwa; Jonas Moecking; Christopher D Goodman; Geoffrey I McFadden; Subathdrage D M Sumanadasa; David P Fairlie; Vicky M Avery; Thomas Kurz; Katherine T Andrews
Journal:  Antimicrob Agents Chemother       Date:  2014-04-14       Impact factor: 5.191

9.  A high-throughput screen targeting malaria transmission stages opens new avenues for drug development.

Authors:  Kathrin Buchholz; Thomas A Burke; Kim C Williamson; Roger C Wiegand; Dyann F Wirth; Matthias Marti
Journal:  J Infect Dis       Date:  2011-05-15       Impact factor: 5.226

10.  Temporal evaluation of commitment to sexual development in Plasmodium falciparum.

Authors:  Christopher L Peatey; Matthew W A Dixon; Donald L Gardiner; Katharine R Trenholme
Journal:  Malar J       Date:  2013-04-22       Impact factor: 2.979
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