BACKGROUND: Effective delivery of photosensitizer to target tumor cells that causes minimal damage to healthy cells is an important requirement in photodynamic therapy of cancer. AIM: We aimed to use biocompatible gold nanoparticles as a vehicle to deliver 5-aminolevulinic (5-ALA) acid for selective and efficient photodynamic therapy. RESULTS: Protoporphyrin IX accumulated preferentially in fibrosarcoma tumor cells treated with 5-ALA-conjugated nanoparticles while yielding significantly higher reactive oxygen species compared with that with 5-ALA. The elevation of reactive oxygen species resulted in 50% more cytotoxicity to tumor cells than that of 5-ALA alone. Selective destruction of tumor cells was also achieved using 5-ALA-conjugated nanoparticles in co-culture with dermal fibroblasts. CONCLUSIONS: 5-ALA-conjugated nanoparticles offer a new modality for selective and efficient destruction of tumor cells, with minimal damage to fibroblasts in the 5-ALA-photodynamic therapy.
BACKGROUND: Effective delivery of photosensitizer to target tumor cells that causes minimal damage to healthy cells is an important requirement in photodynamic therapy of cancer. AIM: We aimed to use biocompatible gold nanoparticles as a vehicle to deliver 5-aminolevulinic (5-ALA) acid for selective and efficient photodynamic therapy. RESULTS:Protoporphyrin IX accumulated preferentially in fibrosarcoma tumor cells treated with 5-ALA-conjugated nanoparticles while yielding significantly higher reactive oxygen species compared with that with 5-ALA. The elevation of reactive oxygen species resulted in 50% more cytotoxicity to tumor cells than that of 5-ALA alone. Selective destruction of tumor cells was also achieved using 5-ALA-conjugated nanoparticles in co-culture with dermal fibroblasts. CONCLUSIONS:5-ALA-conjugated nanoparticles offer a new modality for selective and efficient destruction of tumor cells, with minimal damage to fibroblasts in the 5-ALA-photodynamic therapy.
Authors: Joseph D Meyers; Yu Cheng; Ann-Marie Broome; Richard S Agnes; Mark D Schluchter; Seunghee Margevicius; Xinning Wang; Malcolm E Kenney; Clemens Burda; James P Basilion Journal: Part Part Syst Charact Date: 2015-04 Impact factor: 3.310
Authors: Andrej Babič; V Herceg; E Bastien; H-P Lassalle; L Bezdetnaya; Norbert Lange Journal: Nanoscale Res Lett Date: 2018-01-11 Impact factor: 4.703