Literature DB >> 1902521

Purification, crystallization and preliminary crystallographic analysis of porcine aldose reductase.

O el-Kabbani1, S V Narayana, Y S Babu, K M Moore, T G Flynn, J M Petrash, E M Westbrook, L J DeLucas, C E Bugg.   

Abstract

Large crystals of porcine aldose reductase have been grown from polyethylene glycol solutions. The crystals are triclinic, space-group P1, with a = 81.3 A, b = 85.9 A, c = 56.6 A, alpha = 102.3 degrees, beta = 103.3 degrees and gamma = 79.0 degrees. The crystals grow within ten days to dimensions of 0.6 mm x 0.4 mm x 0.2 mm and diffract to at least 2.5 A. There are four molecules in the unit cell related by a set of three mutually perpendicular non-crystallographic 2-fold axes.

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Year:  1991        PMID: 1902521     DOI: 10.1016/0022-2836(91)90257-7

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  1 in total

1.  Alpha-crystallin-mediated protection of lens cells against heat and oxidative stress-induced cell death.

Authors:  Karen L Christopher; Michelle G Pedler; Biehuoy Shieh; David A Ammar; J Mark Petrash; Niklaus H Mueller
Journal:  Biochim Biophys Acta       Date:  2013-11-22
  1 in total

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