| Literature DB >> 19022294 |
Massimo Pizzato1, Otto Erlwein, David Bonsall, Stephen Kaye, David Muir, Myra O McClure.
Abstract
PCR-enhanced reverse transcriptase assays (PERT) are sensitive tools for the detection of retroviruses in biological samples. The adaptation of real-time PCR techniques based on fluorescent probes (F-PERT) has added a reliable quantitative capacity to the assay. In the interest of economy and time, the SYBR Green I-based real-time detection system was used to establish a convenient one-step PERT assay (SG-PERT). This assay can be completed in 2h, is linear over six orders of magnitude and can be used to quantify retroviruses belonging to divergent species, such as the human immunodeficiency virus type 1 (HIV-1), murine leukemia virus (MLV) and prototypic foamy virus (PFV).Entities:
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Year: 2008 PMID： 19022294 DOI： 10.1016/j.jviromet.2008.10.012
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014