Literature DB >> 19021377

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies.

Han-Wen Guo1, Chien-Tsun Chen, Yau-Huei Wei, Oscar K Lee, Vladimir Gukassyan, Fu-Jen Kao, Hsing-Wen Wang.   

Abstract

The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

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Year:  2008        PMID： 19021377     DOI： 10.1117/1.2990752

Source DB:  PubMed          Journal:  J Biomed Opt        ISSN： 1083-3668            Impact factor:   3.170


  19 in total

1.  Cell death, non-invasively assessed by intrinsic fluorescence intensity of NADH, is a predictive indicator of functional differentiation of embryonic stem cells.

Authors:  David G Buschke; Jayne M Squirrell; Jimmy J Fong; Kevin W Eliceiri; Brenda M Ogle
Journal:  Biol Cell       Date:  2012-03-23       Impact factor: 4.458

2.  Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue.

Authors:  Chiara Stringari; Amanda Cinquin; Olivier Cinquin; Michelle A Digman; Peter J Donovan; Enrico Gratton
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-01       Impact factor: 11.205

3.  Multiphoton flow cytometry to assess intrinsic and extrinsic fluorescence in cellular aggregates: applications to stem cells.

Authors:  David G Buschke; Jayne M Squirrell; Hidayath Ansari; Michael A Smith; Curtis T Rueden; Justin C Williams; Gary E Lyons; Timothy J Kamp; Kevin W Eliceiri; Brenda M Ogle
Journal:  Microsc Microanal       Date:  2010-08-05       Impact factor: 4.127

4.  The metabolic interaction of cancer cells and fibroblasts - coupling between NAD(P)H and FAD, intracellular pH and hydrogen peroxide.

Authors:  Irina N Druzhkova; Marina V Shirmanova; Maria M Lukina; Varvara V Dudenkova; Nataliya M Mishina; Elena V Zagaynova
Journal:  Cell Cycle       Date:  2016-05-02       Impact factor: 4.534

5.  Label-free separation of human embryonic stem cells and their differentiating progenies by phasor fluorescence lifetime microscopy.

Authors:  Chiara Stringari; Robert Sierra; Peter J Donovan; Enrico Gratton
Journal:  J Biomed Opt       Date:  2012-04       Impact factor: 3.170

6.  Phasor fluorescence lifetime microscopy of free and protein-bound NADH reveals neural stem cell differentiation potential.

Authors:  Chiara Stringari; Jamison L Nourse; Lisa A Flanagan; Enrico Gratton
Journal:  PLoS One       Date:  2012-11-05       Impact factor: 3.240

7.  Fluorescence lifetime imaging of alterations to cellular metabolism by domain 2 of the hepatitis C virus core protein.

Authors:  Nirmal Mazumder; Rodney K Lyn; Ragunath Singaravelu; Andrew Ridsdale; Douglas J Moffatt; Chih-Wei Hu; Han-Ruei Tsai; John McLauchlan; Albert Stolow; Fu-Jen Kao; John Paul Pezacki
Journal:  PLoS One       Date:  2013-06-24       Impact factor: 3.240

8.  Metabolic trajectory of cellular differentiation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH.

Authors:  Chiara Stringari; Robert A Edwards; Kira T Pate; Marian L Waterman; Peter J Donovan; Enrico Gratton
Journal:  Sci Rep       Date:  2012-08-10       Impact factor: 4.379

Review 9.  Imaging Approaches in Functional Assessment of Implantable Myogenic Biomaterials and Engineered Muscle Tissue.

Authors:  Kyle J Edmunds; Paolo Gargiulo
Journal:  Eur J Transl Myol       Date:  2015-03-11

10.  Quantitative metabolic imaging using endogenous fluorescence to detect stem cell differentiation.

Authors:  Kyle P Quinn; Gautham V Sridharan; Rebecca S Hayden; David L Kaplan; Kyongbum Lee; Irene Georgakoudi
Journal:  Sci Rep       Date:  2013-12-05       Impact factor: 4.379
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