Literature DB >> 19020819

Analysis of 2-LTR circle junctions of viral DNA in infected cells.

Dibyakanti Mandal1, Vinayaka R Prasad.   

Abstract

The unintegrated viral DNA synthesized during human immunodeficiency virus type 1 infection includes linear and circular forms. Circular forms of viral DNA are surrogate markers for nuclear import of viral DNA during virus replication as well as events surrounding the completion of reverse transcription. Analysis of 2-LTR circles is convenient and the quantity of 2-LTR circle formed is directly proportional to the amount of viral DNA imported into the cell nucleus. In addition, correct synthesis of 2-LTR circles is an outcome of HIV-1 Gag-Pol function. Thus, quantitation and sequence analysis of 2-LTR circles have been very important in studying the structure and function relationship of key viral proteins. In this chapter, we describe the methods of quantitation and analysis of 2-LTR circle junctions isolated from HIV-1 infected cells.

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Year:  2009        PMID： 19020819      PMCID： PMC4151347          DOI： 10.1007/978-1-59745-170-3_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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