Literature DB >> 19020817

Cell-free assays for HIV-1 uncoating.

Christopher Aiken1.   

Abstract

Uncoating is an essential step in the retrovirus life cycle about which little is known. Uncoating is defined as the specific dissociation of the capsid shell from the viral core in the host cell cytoplasm. In this chapter, biochemical assays for studying HIV-1 uncoating in vitro are described. These techniques have proven useful for characterizing HIV-1 mutants that exhibit defects in the uncoating step of infection.

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Mesh：

Year:  2009        PMID： 19020817      PMCID： PMC3842014          DOI： 10.1007/978-1-59745-170-3_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Uncoating of HIV-1 requires cellular activation.

Authors:  Prasert Auewarakul; Pathawut Wacharapornin; Sirawat Srichatrapimuk; Somchai Chutipongtanate; Pilaipan Puthavathana
Journal:  Virology       Date:  2005-06-20       Impact factor: 3.616

2.  Properties of avian retrovirus particles defective in viral protease.

Authors:  L Stewart; G Schatz; V M Vogt
Journal:  J Virol       Date:  1990-10       Impact factor: 5.103

3.  p24 antigen capture assay for quantification of human immunodeficiency virus using readily available inexpensive reagents.

Authors:  K Wehrly; B Chesebro
Journal:  Methods       Date:  1997-08       Impact factor: 3.608

4.  The preparation and biochemical characterization of intact capsids of equine infectious anemia virus.

Authors:  M M Roberts; S Oroszlan
Journal:  Biochem Biophys Res Commun       Date:  1989-04-28       Impact factor: 3.575

5.  Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.

Authors:  A Adachi; H E Gendelman; S Koenig; T Folks; R Willey; A Rabson; M A Martin
Journal:  J Virol       Date:  1986-08       Impact factor: 5.103

6.  Identification and isolation of the major core protein from the oncornavirus-like particle in human milk.

Authors:  P Furmanski; C P Loeckner; C Longley; L J Larson; M A Rich
Journal:  Cancer Res       Date:  1976-11       Impact factor: 12.701

7.  Characteristics of cores of avian leuko-sarcoma viruses.

Authors:  J P Bader; N R Brown; A V Bader
Journal:  Virology       Date:  1970-08       Impact factor: 3.616

8.  Recovery of antigenically reactive HIV-2 cores.

Authors:  I L Chrystie; J D Almeida
Journal:  J Med Virol       Date:  1989-03       Impact factor: 2.327

9.  Formation of a human immunodeficiency virus type 1 core of optimal stability is crucial for viral replication.

Authors:  Brett M Forshey; Uta von Schwedler; Wesley I Sundquist; Christopher Aiken
Journal:  J Virol       Date:  2002-06       Impact factor: 5.103

10.  Isolation of human immunodeficiency virus type 1 cores: retention of Vpr in the absence of p6(gag).

Authors:  M A Accola; A Ohagen; H G Göttlinger
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103
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  23 in total

1.  A mutant tat protein inhibits HIV-1 reverse transcription by targeting the reverse transcription complex.

Authors:  Min-Hsuan Lin; Ann Apolloni; Vincent Cutillas; Haran Sivakumaran; Sally Martin; Dongsheng Li; Ting Wei; Rui Wang; Hongping Jin; Kirsten Spann; David Harrich
Journal:  J Virol       Date:  2015-02-11       Impact factor: 5.103

2.  A Novel Phenotype Links HIV-1 Capsid Stability to cGAS-Mediated DNA Sensing.

Authors:  Mohammad Adnan Siddiqui; Akatsuki Saito; Upul D Halambage; Damien Ferhadian; Douglas K Fischer; Ashwanth C Francis; Gregory B Melikyan; Zandrea Ambrose; Christopher Aiken; Masahiro Yamashita
Journal:  J Virol       Date:  2019-07-30       Impact factor: 5.103

3.  PF74 Inhibits HIV-1 Integration by Altering the Composition of the Preintegration Complex.

Authors:  Muthukumar Balasubramaniam; Jing Zhou; Amma Addai; Phillip Martinez; Jui Pandhare; Christopher Aiken; Chandravanu Dash
Journal:  J Virol       Date:  2019-03-05       Impact factor: 5.103

4.  In vitro uncoating of HIV-1 cores.

Authors:  Vaibhav B Shah; Christopher Aiken
Journal:  J Vis Exp       Date:  2011-11-08       Impact factor: 1.355

5.  Novel postentry inhibitor of human immunodeficiency virus type 1 replication screened by yeast membrane-associated two-hybrid system.

Authors:  Emiko Urano; Noriko Kuramochi; Reiko Ichikawa; Somay Yamagata Murayama; Kosuke Miyauchi; Hiroshi Tomoda; Yutaka Takebe; Milan Nermut; Jun Komano; Yuko Morikawa
Journal:  Antimicrob Agents Chemother       Date:  2011-07-11       Impact factor: 5.191

6.  Identification of capsid mutations that alter the rate of HIV-1 uncoating in infected cells.

Authors:  Amy E Hulme; Z Kelley; Eneniziaogochukwu A Okocha; Thomas J Hope
Journal:  J Virol       Date:  2014-10-22       Impact factor: 5.103

7.  The cyclosporin A washout assay to detect HIV-1 uncoating in infected cells.

Authors:  Amy E Hulme; Thomas J Hope
Journal:  Methods Mol Biol       Date:  2014

8.  CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5α.

Authors:  Gongpu Zhao; Peijun Zhang
Journal:  Methods Mol Biol       Date:  2014

9.  Small-molecule inhibition of human immunodeficiency virus type 1 infection by virus capsid destabilization.

Authors:  Jiong Shi; Jing Zhou; Vaibhav B Shah; Christopher Aiken; Kevin Whitby
Journal:  J Virol       Date:  2010-10-20       Impact factor: 5.103

10.  The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b.

Authors:  Evan Chaudhuri; Sabyasachi Dash; Muthukumar Balasubramaniam; Adrian Padron; Joseph Holland; Gregory A Sowd; Fernando Villalta; Alan N Engelman; Jui Pandhare; Chandravanu Dash
Journal:  J Biol Chem       Date:  2020-03-09       Impact factor: 5.157
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