BACKGROUND: Molecular methods were used to characterize influenza A (H1N1) and (H3N2) strains and to identify amantadine-resistance. OBJECTIVES: To compare proportions of amantadine-resistant influenza A (H1N1) and (H3N2) isolates in the Greater Toronto Area. STUDY DESIGN: Isolates of influenza A (H1N1) and (H3N2) were strain typed using molecular methods. Pyrosequencing for point mutations in the transmembrane domain of the M2 proton channel was undertaken. Proportions of amantadine-resistant and susceptible isolates were compared using the The Fisher's exact test. RESULTS: 96% of the 49 influenza A (H3N2) isolates and none of the influenza A (H1N1) tested carried a point mutation in the M gene coding for the M2 protein. Influenza A (H3N2) isolates were more likely to carry an amantadine-resistance associated mutation than influenza A (H1N1) isolates (Fishers's exact test, P<0.0001). CONCLUSIONS: : Characterization of amantadine-resistance in influenza A (H1N1) isolates should utilize a variety of different methods including sub-typing, strain typing, and direct sequencing for point mutations associated with amantadine-resistance.
BACKGROUND: Molecular methods were used to characterize influenza A (H1N1) and (H3N2) strains and to identify amantadine-resistance. OBJECTIVES: To compare proportions of amantadine-resistant influenza A (H1N1) and (H3N2) isolates in the Greater Toronto Area. STUDY DESIGN: Isolates of influenza A (H1N1) and (H3N2) were strain typed using molecular methods. Pyrosequencing for point mutations in the transmembrane domain of the M2 proton channel was undertaken. Proportions of amantadine-resistant and susceptible isolates were compared using the The Fisher's exact test. RESULTS: 96% of the 49 influenza A (H3N2) isolates and none of the influenza A (H1N1) tested carried a point mutation in the M gene coding for the M2 protein. Influenza A (H3N2) isolates were more likely to carry an amantadine-resistance associated mutation than influenza A (H1N1) isolates (Fishers's exact test, P<0.0001). CONCLUSIONS: : Characterization of amantadine-resistance in influenza A (H1N1) isolates should utilize a variety of different methods including sub-typing, strain typing, and direct sequencing for point mutations associated with amantadine-resistance.
Authors: Jason J LeBlanc; Yan Li; Nathalie Bastien; Kevin R Forward; Ross J Davidson; Todd F Hatchette Journal: J Clin Microbiol Date: 2009-09-30 Impact factor: 5.948
Authors: Muthannan A Ramakrishnan; Zheng Jin Tu; Sushmita Singh; Ashok K Chockalingam; Marie R Gramer; Ping Wang; Sagar M Goyal; My Yang; David A Halvorson; Srinand Sreevatsan Journal: PLoS One Date: 2009-09-22 Impact factor: 3.240