J-C Tang1, H Taniguchi, H Chu, Q Zhou, S Nagata. 1. Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education /College of Environmental Science and Engineering, Nankai University, Tianjin, China. jc_tang@163.com
Abstract
AIMS: Isolation of novel alginate degrading bacteria for the disposal of seaweed waste in composting process. METHODS AND RESULTS: Decomposition of alginate polymers was checked by the 3,5-dinitrosalicylic acid (DNS) method for reducing sugar, and absorbance at 235 nm for unsaturated sugar. A bacterium A7 was isolated from wakame compost and confirmed to belong to the genus Gracilibacillus by partial 16S rDNA analysis. The optimum condition for the growth of A7 in a medium containing 5 g l(-1) of sodium alginate is as follows: pH, 8.5-9.5; NaCl, 0.5 mol l(-1); temperature, 30 degrees C and polypeptone as nutrient content, 2-5 g l(-1). In a laboratory-scale composting experiment, the alginate content in wakame compost decreased to 14.3% after 72 h of composting from an initial value of 36%, indicating the effectiveness of alginate decomposition of A7 in wakame composting. CONCLUSIONS: The bacterium A7 was found to be alginate lyase-producing in genus Gracilibacillus and effective in degrading alginate to oligosaccharides in wakame during composting process. SIGNIFICANCE AND IMPACT OF THE STUDY: Development of new methods for the disposal of marine wastes and production of functional products.
AIMS: Isolation of novel alginate degrading bacteria for the disposal of seaweed waste in composting process. METHODS AND RESULTS: Decomposition of alginate polymers was checked by the 3,5-dinitrosalicylic acid (DNS) method for reducing sugar, and absorbance at 235 nm for unsaturated sugar. A bacterium A7 was isolated from wakame compost and confirmed to belong to the genus Gracilibacillus by partial 16S rDNA analysis. The optimum condition for the growth of A7 in a medium containing 5 g l(-1) of sodium alginate is as follows: pH, 8.5-9.5; NaCl, 0.5 mol l(-1); temperature, 30 degrees C and polypeptone as nutrient content, 2-5 g l(-1). In a laboratory-scale composting experiment, the alginate content in wakame compost decreased to 14.3% after 72 h of composting from an initial value of 36%, indicating the effectiveness of alginate decomposition of A7 in wakame composting. CONCLUSIONS: The bacterium A7 was found to be alginate lyase-producing in genus Gracilibacillus and effective in degrading alginate to oligosaccharides in wakame during composting process. SIGNIFICANCE AND IMPACT OF THE STUDY: Development of new methods for the disposal of marine wastes and production of functional products.