Isolation and confirmation of Yersinia pestis mutants exempt from select agent regulations.

This unit describes protocols for Yersinia pestis to confirm plasmid profiles, construct and confirm a Deltapgm mutation, and cure the low-calcium response (Lcr) plasmid encoding a type III secretion system (TTSS). Strains lacking either the chromosomal pgm locus or the Lcr plasmid can be safely studied under BSL-2 conditions and are exempt from Select Agent regulations in the U.S. Copyright 2008 by John Wiley & Sons, Inc.