PURPOSE: The purpose of this study was to modify the method of platelet-rich plasma (PRP) preparation for obtaining optimal angiogenic potential and accelerate bone healing. Also, the potential synergistic effect of a suboptimal concentration of bone morphogenic protein-2 (BMP-2) and modified PRP (mPRP) on bone healing was evaluated in vivo. MATERIALS AND METHODS: The angiogenic factor-enriched PRP, which included peripheral blood mononuclear cells (mostly lymphocytes and monocytes, excluding polymorphonuclear leukocytes [PMNs], was achieved by lowering concentrations of thrombin and CaCl2, after pre-activation with shear stress using a table-top vortex machine and collagen. In vitro, endothelial cell migration activity in the mPRP group was compared to conventional PRP preparation using a modified Boyden chamber assay. In an animal study, PGA scaffold, PGA scaffold + mPRP, PGA scaffold + mPRP + rhBMP-2, and PGA scaffold + rhBMP-2 were applied to critical-sized calvarial defects in 28 nude rats. At 2 weeks, periosteal blood flow was measured using laser Doppler perfusion imaging, and bone formation was evaluated at 8 weeks by histology, dual energy x-ray absorptiometry, and micro-computed tomography. RESULTS: mPRP induced faster migration of cord blood-derived outgrowth endothelial-like cells. In vivo, the group with mPRP with a low dose of rhBMP-2 showed significantly increased numbers of blood vessels at 2 weeks and notable synergistic effect on bone healing at 8 weeks as evaluated with histology, bone mineral density and bone mineral content, and muCT. CONCLUSION: The mPRP used in this study improved vascular perfusion around the defect and resulted in enhanced bone healing. Also, combining mPRP with a suboptimal dosage of rhBMP-2 improved bone formation and enhanced bone density.
PURPOSE: The purpose of this study was to modify the method of platelet-rich plasma (PRP) preparation for obtaining optimal angiogenic potential and accelerate bone healing. Also, the potential synergistic effect of a suboptimal concentration of bone morphogenic protein-2 (BMP-2) and modified PRP (mPRP) on bone healing was evaluated in vivo. MATERIALS AND METHODS: The angiogenic factor-enriched PRP, which included peripheral blood mononuclear cells (mostly lymphocytes and monocytes, excluding polymorphonuclear leukocytes [PMNs], was achieved by lowering concentrations of thrombin and CaCl2, after pre-activation with shear stress using a table-top vortex machine and collagen. In vitro, endothelial cell migration activity in the mPRP group was compared to conventional PRP preparation using a modified Boyden chamber assay. In an animal study, PGA scaffold, PGA scaffold + mPRP, PGA scaffold + mPRP + rhBMP-2, and PGA scaffold + rhBMP-2 were applied to critical-sized calvarial defects in 28 nude rats. At 2 weeks, periosteal blood flow was measured using laser Doppler perfusion imaging, and bone formation was evaluated at 8 weeks by histology, dual energy x-ray absorptiometry, and micro-computed tomography. RESULTS:mPRP induced faster migration of cord blood-derived outgrowth endothelial-like cells. In vivo, the group with mPRP with a low dose of rhBMP-2 showed significantly increased numbers of blood vessels at 2 weeks and notable synergistic effect on bone healing at 8 weeks as evaluated with histology, bone mineral density and bone mineral content, and muCT. CONCLUSION: The mPRP used in this study improved vascular perfusion around the defect and resulted in enhanced bone healing. Also, combining mPRP with a suboptimal dosage of rhBMP-2 improved bone formation and enhanced bone density.
Authors: Y Miyazaki; S Nomura; T Miyake; H Kagawa; C Kitada; H Taniguchi; Y Komiyama; Y Fujimura; Y Ikeda; S Fukuhara Journal: Blood Date: 1996-11-01 Impact factor: 22.113
Authors: Yen-Chen Huang; Darnell Kaigler; Kevin G Rice; Paul H Krebsbach; David J Mooney Journal: J Bone Miner Res Date: 2004-12-20 Impact factor: 6.741
Authors: Benjamin Levi; Aaron W James; Emily R Nelson; Michelle Peng; Derrick C Wan; George W Commons; Min Lee; Benjamin Wu; Michael T Longaker Journal: Plast Reconstr Surg Date: 2011-03 Impact factor: 4.730
Authors: Benjamin Dallaudière; Marta Lempicki; Lionel Pesquer; Liliane Louedec; Pierre Marie Preux; Philippe Meyer; Vincent Hummel; Ahmed Larbi; Lydia Deschamps; Clement Journe; Agathe Hess; Alain Silvestre; Paul Sargos; Philippe Loriaut; Patrick Boyer; Elisabeth Schouman-Claeys; Jean Baptiste Michel; Jean Michel Serfaty Journal: Eur Radiol Date: 2013-06-26 Impact factor: 5.315
Authors: Benjamin Dallaudiere; Liliane Louedec; Marie Paule Jacob Lenet; Lionel Pesquer; Elvind Blaise; Anne Perozziello; Jean Baptiste Michel; Maryse Moinard; Philippe Meyer; Jean Michel Serfaty Journal: Muscles Ligaments Tendons J Date: 2015-07-03