OBJECTIVE: To report a successful pregnancy after intracytoplasmic sperm injection (ICSI) with artificial oocyte activation (AOA) on a patient whose fertilization rate after ICSI was extremely low; and to report on cytologic analyses of the fertilization failure (FF) eggs after ICSI and a biologic assessment of the sperm of this patient. DESIGN: Case report with an assessment of gamete function. SETTING: University hospital and an experimental laboratory. PATIENT(S): A couple with severe oligoasthenozoospermia, whose seventh attempt at ICSI ended in the failure. INTERVENTION(S): Cytologic analyses of FF eggs after ICSI, AOA after ICSI, and analyses of human sperm oocyte activation ability and centrosomal function. RESULT(S): Fertilization arrest after ICSI was observed in FF eggs at various stages of fertilization. After artificial oocyte activation by exposure to ionomycin, clinical pregnancy was confirmed, and a healthy baby was born. As assessed by heterologous ICSI of human sperm into bovine oocytes, there was no significant difference in the oocyte activation rates between the patient's and control sperm, but the sperm centrosomal function was low in the patient's sperm (48.5% vs. 69.6%). CONCLUSION(S): We report a successful pregnancy after ICSI with AOA using a calcium ionophore, after critical cytologic analyses of the FF eggs. Furthermore, sperm centrosomal function was low, indicating that sperm's ability to process the events of fertilization after the oocyte activation was poor in this patient.
OBJECTIVE: To report a successful pregnancy after intracytoplasmic sperm injection (ICSI) with artificial oocyte activation (AOA) on a patient whose fertilization rate after ICSI was extremely low; and to report on cytologic analyses of the fertilization failure (FF) eggs after ICSI and a biologic assessment of the sperm of this patient. DESIGN: Case report with an assessment of gamete function. SETTING: University hospital and an experimental laboratory. PATIENT(S): A couple with severe oligoasthenozoospermia, whose seventh attempt at ICSI ended in the failure. INTERVENTION(S): Cytologic analyses of FF eggs after ICSI, AOA after ICSI, and analyses of human sperm oocyte activation ability and centrosomal function. RESULT(S): Fertilization arrest after ICSI was observed in FF eggs at various stages of fertilization. After artificial oocyte activation by exposure to ionomycin, clinical pregnancy was confirmed, and a healthy baby was born. As assessed by heterologous ICSI of human sperm into bovine oocytes, there was no significant difference in the oocyte activation rates between the patient's and control sperm, but the sperm centrosomal function was low in the patient's sperm (48.5% vs. 69.6%). CONCLUSION(S): We report a successful pregnancy after ICSI with AOA using a calcium ionophore, after critical cytologic analyses of the FF eggs. Furthermore, sperm centrosomal function was low, indicating that sperm's ability to process the events of fertilization after the oocyte activation was poor in this patient.
Authors: Hoi Chang Lee; Margaret Arny; Daniel Grow; Daniel Dumesic; Rafael A Fissore; Teru Jellerette-Nolan Journal: J Assist Reprod Genet Date: 2014-04-23 Impact factor: 3.412
Authors: Katerina A Turner; Emily L Fishman; Mariam Asadullah; Brooke Ott; Patrick Dusza; Tariq A Shah; Puneet Sindhwani; Nagalakshmi Nadiminty; Emanuela Molinari; Pasquale Patrizio; Barbara S Saltzman; Tomer Avidor-Reiss Journal: Front Cell Dev Biol Date: 2021-04-22
Authors: Mehri Makki; Elham Saboori; Mohammad Ali Sabbaghi; Raheleh Aram; Mohammad Hasan Fallahian; Fatemeh Peyghambari; Hessam Roustaei; Abbas Ahmadi Journal: Iran J Reprod Med Date: 2012-07