RATIONALE: Human secretory leukocyte protease inhibitor (SLPI) displays bactericidal activity against pathogens such as Escherichia coli and Streptococcus. Furthermore, it has been reported that murine SLPI shows potent antimycobacterial activity. OBJECTIVES: The aim of the present study was to investigate whether human recombinant SLPI not only kills mycobacteria but also acts as a pattern recognition receptor for the host immune system. METHODS: For the in vivo experiment, BALB/c mice were infected by intranasal instillation with Mycobacterium bovis BCG and viable BCG load in lung homogenates was later determined. For the in vitro experiments, SLPI was incubated overnight with a suspension of M. bovis BCG or the virulent strain Mycobacterium tuberculosis H37Rv, and the percentage survival as well as the binding of SLPI to mycobacteria was determined. Furthermore, bacteria phagocytosis was also determined by flow cytometry. MEASUREMENTS AND MAIN RESULTS: Intranasal SLPI treatment decreased the number of colony-forming units recovered from lung homogenates, indicating that SLPI interfered with M. bovis BCG infection. Moreover, SLPI decreased the viability of both M. bovis BCG and H37Rv. We demonstrated that SLPI attached to the surface of the mycobacteria by binding to pathogen-associated molecular pattern mannan-capped lipoarabinomannans and phosphatidylinositol mannoside. Furthermore, we found that in the sputum of patients with tuberculosis, mycobacteria were coated with endogenous SLPI. Finally, we showed that phagocytosis of SLPI-coated mycobacteria was faster than that of uncoated bacteria. CONCLUSIONS: The present results demonstrate for the first time that human SLPI kills mycobacteria and is a new pattern recognition receptor for them.
RATIONALE: Humansecretory leukocyte protease inhibitor (SLPI) displays bactericidal activity against pathogens such as Escherichia coli and Streptococcus. Furthermore, it has been reported that murineSLPI shows potent antimycobacterial activity. OBJECTIVES: The aim of the present study was to investigate whether human recombinant SLPI not only kills mycobacteria but also acts as a pattern recognition receptor for the host immune system. METHODS: For the in vivo experiment, BALB/c mice were infected by intranasal instillation with Mycobacterium bovis BCG and viable BCG load in lung homogenates was later determined. For the in vitro experiments, SLPI was incubated overnight with a suspension of M. bovis BCG or the virulent strain Mycobacterium tuberculosis H37Rv, and the percentage survival as well as the binding of SLPI to mycobacteria was determined. Furthermore, bacteria phagocytosis was also determined by flow cytometry. MEASUREMENTS AND MAIN RESULTS: Intranasal SLPI treatment decreased the number of colony-forming units recovered from lung homogenates, indicating that SLPI interfered with M. bovis BCG infection. Moreover, SLPI decreased the viability of both M. bovis BCG and H37Rv. We demonstrated that SLPI attached to the surface of the mycobacteria by binding to pathogen-associated molecular pattern mannan-capped lipoarabinomannans and phosphatidylinositolmannoside. Furthermore, we found that in the sputum of patients with tuberculosis, mycobacteria were coated with endogenous SLPI. Finally, we showed that phagocytosis of SLPI-coated mycobacteria was faster than that of uncoated bacteria. CONCLUSIONS: The present results demonstrate for the first time that humanSLPI kills mycobacteria and is a new pattern recognition receptor for them.
Authors: Diego Guerrieri; Nancy L Tateosian; Paulo C Maffía; Romina M Reiteri; Nicolás O Amiano; María J Costa; Ximena Villalonga; Mercedes L Sanchez; Silvia M Estein; Verónica E Garcia; Jean-Michel Sallenave; Héctor E Chuluyan Journal: Immunology Date: 2011-05-17 Impact factor: 7.397
Authors: Nancy L Tateosian; Virginia Pasquinelli; Rodrigo E Hernández Del Pino; Nella Ambrosi; Diego Guerrieri; Sigifredo Pedraza-Sánchez; Natalia Santucci; Luciano D'Attilio; Joaquín Pellegrini; María A Araujo-Solis; Rosa M Musella; Domingo J Palmero; Rogelio Hernandez-Pando; Verónica E Garcia; H Eduardo Chuluyan Journal: Am J Pathol Date: 2014-03-04 Impact factor: 4.307
Authors: Galina V Mukamolova; Obolbek Turapov; Joanne Malkin; Gerrit Woltmann; Michael R Barer Journal: Am J Respir Crit Care Med Date: 2009-10-29 Impact factor: 21.405
Authors: Zhiguo Li; Allison Moy; Kirti Sohal; Carolyn Dam; Peter Kuo; James Whittaker; Mei Whittaker; Nejat Düzgünes; Krystyna Konopka; Andreas H Franz; Joan Lin-Cereghino; Geoff P Lin-Cereghino Journal: Protein Expr Purif Date: 2009-06-06 Impact factor: 1.650