Ling Zhang1, Yan Wang, Ai-Hua Liao. 1. Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, China.
Abstract
OBJECTIVE: To determine if quantitative abnormalities of circulating fetal trophoblast cells (CFTCs) are associated with preeclampsia. METHODS: The trophoblast cell-specific antibody, MEM-G/9 (monoclonal antibody to HLA-G), was applied to distinguish the trophoblast cells from the maternal circulation. The trophoblast cells were isolated by density-gradient centrifugation from maternal blood samples of normal pregnant and preeclamptic women, respectively. After preliminary enrichment, the CFTCs were identified by immunocytochemical staining with the MEM-G/9. To prove fetal origin of the HLA-G-positive cells, primer-extension preamplification (PEP) and polymerase chain reaction (PCR) based on single HLA-G-positive cells were adopted to detect human sex-determining region of the Y-chromosome (SRY) gene. RESULTS: There were 6.88 +/- 1.54 and 30.56 +/- 5.16 HLA-G-positive cells in 6 mL maternal blood from the normal pregnant (n = 16) and preeclamptic women (n = 18), respectively. The difference was statistically significant (p < 0.001). The SRY gene from the HLA-G-positive cells was detected in all pregnant women carrying male fetuses. The sensitivity and specificity of PEP and PCR for the SRY gene detection were 100%. CONCLUSION: It is concluded that enhancement of CFTCs numbers is related to preeclampsia, which provides information useful for noninvasive prenatal diagnosis of preeclampsia. Copyright (c) 2008 John Wiley & Sons, Ltd.
OBJECTIVE: To determine if quantitative abnormalities of circulating fetal trophoblast cells (CFTCs) are associated with preeclampsia. METHODS: The trophoblast cell-specific antibody, MEM-G/9 (monoclonal antibody to HLA-G), was applied to distinguish the trophoblast cells from the maternal circulation. The trophoblast cells were isolated by density-gradient centrifugation from maternal blood samples of normal pregnant and preeclamptic women, respectively. After preliminary enrichment, the CFTCs were identified by immunocytochemical staining with the MEM-G/9. To prove fetal origin of the HLA-G-positive cells, primer-extension preamplification (PEP) and polymerase chain reaction (PCR) based on single HLA-G-positive cells were adopted to detect human sex-determining region of the Y-chromosome (SRY) gene. RESULTS: There were 6.88 +/- 1.54 and 30.56 +/- 5.16 HLA-G-positive cells in 6 mL maternal blood from the normal pregnant (n = 16) and preeclamptic women (n = 18), respectively. The difference was statistically significant (p < 0.001). The SRY gene from the HLA-G-positive cells was detected in all pregnant women carrying male fetuses. The sensitivity and specificity of PEP and PCR for the SRY gene detection were 100%. CONCLUSION: It is concluded that enhancement of CFTCs numbers is related to preeclampsia, which provides information useful for noninvasive prenatal diagnosis of preeclampsia. Copyright (c) 2008 John Wiley & Sons, Ltd.
Authors: Amy M Breman; Jennifer C Chow; Lance U'Ren; Elizabeth A Normand; Sadeem Qdaisat; Li Zhao; David M Henke; Rui Chen; Chad A Shaw; Laird Jackson; Yaping Yang; Liesbeth Vossaert; Rachel H V Needham; Elizabeth J Chang; Daniel Campton; Jeffrey L Werbin; Ron C Seubert; Ignatia B Van den Veyver; Jackie L Stilwell; Eric P Kaldjian; Arthur L Beaudet Journal: Prenat Diagn Date: 2016-10-02 Impact factor: 3.050