OBJECTIVE: To identify candidate biomarkers for squamous cervical cancer as well as reveal the molecular mechanism underlying this disease by a proteomic approach. METHODS: Proteins from 10 pairs of human squamous cervical cancer and matching adjacent normal cervical tissues were separated by two-dimensional gel electrophoresis and the differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Then, some of the interesting proteins obtained were confirmed by Western blotting in the other 20 pairs of tissues. RESULTS: A comparison of protein patterns revealed 55 protein spots significantly changed, of which 24 protein spots with concordantly increased and 31 protein spots with concordantly decreased intensity in squamous cervical cancer compared with adjacent normal cervical tissues. Thirty-two of these proteins were identified by mass spectrometry. The overexpression of the Tyk2, S100A9, and Zinc finger protein 217 in squamous cervical cancer was confirmed by immunoblotting. CONCLUSIONS: Our study suggested that a proteomics-based approach is useful for developing a more complete picture of the protein profile of squamous cervical cancer. Further ongoing analysis of these differential proteins will determine their potential applicability to squamous cervical cancer-specific diagnosis and therapeutics.
OBJECTIVE: To identify candidate biomarkers for squamous cervical cancer as well as reveal the molecular mechanism underlying this disease by a proteomic approach. METHODS: Proteins from 10 pairs of humansquamous cervical cancer and matching adjacent normal cervical tissues were separated by two-dimensional gel electrophoresis and the differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Then, some of the interesting proteins obtained were confirmed by Western blotting in the other 20 pairs of tissues. RESULTS: A comparison of protein patterns revealed 55 protein spots significantly changed, of which 24 protein spots with concordantly increased and 31 protein spots with concordantly decreased intensity in squamous cervical cancer compared with adjacent normal cervical tissues. Thirty-two of these proteins were identified by mass spectrometry. The overexpression of the Tyk2, S100A9, and Zinc finger protein 217 in squamous cervical cancer was confirmed by immunoblotting. CONCLUSIONS: Our study suggested that a proteomics-based approach is useful for developing a more complete picture of the protein profile of squamous cervical cancer. Further ongoing analysis of these differential proteins will determine their potential applicability to squamous cervical cancer-specific diagnosis and therapeutics.
Authors: Mohd Altaf Najar; Mohammad Arefian; David Sidransky; Harsha Gowda; T S Keshava Prasad; Prashant Kumar Modi; Aditi Chatterjee Journal: Front Genet Date: 2022-05-13 Impact factor: 4.772
Authors: Enoc M Cortés-Malagón; José Bonilla-Delgado; José Díaz-Chávez; Alfredo Hidalgo-Miranda; Sandra Romero-Cordoba; Aykut Uren; Haydar Celik; Matthew McCormick; José A Munguía-Moreno; Eloisa Ibarra-Sierra; Jaime Escobar-Herrera; Paul F Lambert; Daniel Mendoza-Villanueva; Rosa M Bermudez-Cruz; Patricio Gariglio Journal: Virology Date: 2013-09-27 Impact factor: 3.616
Authors: M I Lomnytska; S Becker; I Bodin; A Olsson; K Hellman; A-C Hellström; M Mints; U Hellman; G Auer; S Andersson Journal: Br J Cancer Date: 2010-11-30 Impact factor: 7.640