INTRODUCTION: Tissue factor (TF) pathway inhibitor (TFPI) is the endogenous inhibitor regulating TF-induced blood coagulation. Several polymorphisms have been identified in the TFPI gene and some of them have been correlated with variations in plasma TFPI levels. The aim of the present study was to characterize the TFPI(V264M) mutant in comparison with the wild type protein (TFPI(WT)). MATERIALS AND METHODS: We have overexpressed the TFPI(V264M) mutant and TFPI(WT) in human coronary artery endothelial cells and compared the expression and activity levels of the mutated protein relative to the TFPI(WT). The protein levels were determined by ELISA, the inhibitory activity of the proteins was assessed with a chromogenic substrate assay. The mRNA level of the two TFPI variants was determined using real time RT-PCR. MFOLD was used to predict mRNA secondary structure. RESULTS AND CONCLUSIONS: TFPI(V264M) displayed increased protein levels and activity compared to TFPI(WT) accompanied by an increase in mRNA levels of TFPI(V264M) due to prolonged stability of TFPI(V264M) mRNA. The specific activity of the TFPI(V264M) was similar to TFPI(WT), indicating that the mutation does not affect the enzymatic function of the protein.
INTRODUCTION:Tissue factor (TF) pathway inhibitor (TFPI) is the endogenous inhibitor regulating TF-induced blood coagulation. Several polymorphisms have been identified in the TFPI gene and some of them have been correlated with variations in plasma TFPI levels. The aim of the present study was to characterize the TFPI(V264M) mutant in comparison with the wild type protein (TFPI(WT)). MATERIALS AND METHODS: We have overexpressed the TFPI(V264M) mutant and TFPI(WT) in human coronary artery endothelial cells and compared the expression and activity levels of the mutated protein relative to the TFPI(WT). The protein levels were determined by ELISA, the inhibitory activity of the proteins was assessed with a chromogenic substrate assay. The mRNA level of the two TFPI variants was determined using real time RT-PCR. MFOLD was used to predict mRNA secondary structure. RESULTS AND CONCLUSIONS:TFPI(V264M) displayed increased protein levels and activity compared to TFPI(WT) accompanied by an increase in mRNA levels of TFPI(V264M) due to prolonged stability of TFPI(V264M) mRNA. The specific activity of the TFPI(V264M) was similar to TFPI(WT), indicating that the mutation does not affect the enzymatic function of the protein.