Literature DB >> 1900697

A method for sequencing polymerase chain reaction products can be used to sequence Bacillus subtilis "miniprep" plasmid DNA.

D H Bechhofer1.   

Abstract

Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for polymerase chain reaction product sequencing, it was found that this protocol could be used for sequencing plasmid DNA isolated from Bacillus subtilis.

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Year:  1991        PMID： 1900697

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  6 in total

1.  Regulation of the macrolide-lincosamide-streptogramin B resistance gene ermD.

Authors:  K K Hue; D H Bechhofer
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

2.  Effect of ermC leader region mutations on induced mRNA stability.

Authors:  K K Hue; D H Bechhofer
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Organization and nucleotide sequence of the atp genes encoding the ATP synthase from alkaliphilic Bacillus firmus OF4.

Authors:  D M Ivey; T A Krulwich
Journal:  Mol Gen Genet       Date:  1991-10

4.  Thermal cycle dideoxy DNA sequencing.

Authors:  B E Slatko
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

5.  Rapid isolation and sequencing of purified plasmid DNA from Bacillus subtilis.

Authors:  M I Voskuil; G H Chambliss
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792

6.  Selection of antigen-specific, idiotype-positive B cells in transgenic mice expressing a rearranged M167-mu heavy chain gene.

Authors:  J J Kenny; C O'Connell; D G Sieckmann; R T Fischer; D L Longo
Journal:  J Exp Med       Date:  1991-11-01       Impact factor: 14.307
  6 in total

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