OBJECTIVE: To describe success rates with bipronuclear (2PN) oocyte cryopreservation, followed by thaw, extended culture, and blastocyst transfer. DESIGN: Retrospective study. SETTING: Private fertility center. PATIENT(S): There were 48 blastocyst transfers after post-thaw extended culture in patients less than 35 years old and 43 transfers in patients 35-40 years old. INTERVENTION(S): Patients opted for cryopreservation of their entire cohorts at the 2PN stage. Thawed 2PN oocytes were cultured to the blastocyst stage before transfer. MAIN OUTCOME MEASURE(S): Implantation, ongoing pregnancy. RESULT(S): Among patients less than 35 years old at oocyte retrieval, there were 59 thaws of 2PN oocytes, 48 blastocyst transfers, and 40 ongoing pregnancies (79.2% per transfer), the implantation rate was 64.2%, and the ongoing pregnancy rate (PR) per thaw was 64.4%. Among patients 35-40 years of age at retrieval, there were 58 thaws, 43 blastocyst transfers, and 22 ongoing pregnancies (51.2% per transfer), the implantation rate was 44.0%, and the ongoing PR per thaw was 39.3%. Patients less than 35 years old had significantly greater rates of implantation and ongoing pregnancy than did patients 35-40 years of age. Patients with 12 or more 2PN oocytes had significantly greater rate of ongoing pregnancy than those with fewer than 12 2PN oocytes. CONCLUSION(S): Post-thaw extended culture and blastocyst transfer yield high rates of implantation and ongoing pregnancy. Ongoing PRs decline with increasing age.
OBJECTIVE: To describe success rates with bipronuclear (2PN) oocyte cryopreservation, followed by thaw, extended culture, and blastocyst transfer. DESIGN: Retrospective study. SETTING: Private fertility center. PATIENT(S): There were 48 blastocyst transfers after post-thaw extended culture in patients less than 35 years old and 43 transfers in patients 35-40 years old. INTERVENTION(S): Patients opted for cryopreservation of their entire cohorts at the 2PN stage. Thawed 2PN oocytes were cultured to the blastocyst stage before transfer. MAIN OUTCOME MEASURE(S): Implantation, ongoing pregnancy. RESULT(S): Among patients less than 35 years old at oocyte retrieval, there were 59 thaws of 2PN oocytes, 48 blastocyst transfers, and 40 ongoing pregnancies (79.2% per transfer), the implantation rate was 64.2%, and the ongoing pregnancy rate (PR) per thaw was 64.4%. Among patients 35-40 years of age at retrieval, there were 58 thaws, 43 blastocyst transfers, and 22 ongoing pregnancies (51.2% per transfer), the implantation rate was 44.0%, and the ongoing PR per thaw was 39.3%. Patients less than 35 years old had significantly greater rates of implantation and ongoing pregnancy than did patients 35-40 years of age. Patients with 12 or more 2PN oocytes had significantly greater rate of ongoing pregnancy than those with fewer than 12 2PN oocytes. CONCLUSION(S): Post-thaw extended culture and blastocyst transfer yield high rates of implantation and ongoing pregnancy. Ongoing PRs decline with increasing age.
Authors: Luis Guzman; D Nuñez; R López; N Inoue; J Portella; F Vizcarra; L Noriega-Portella; L Noriega-Hoces; S Munné Journal: J Assist Reprod Genet Date: 2018-10-17 Impact factor: 3.412
Authors: Felipe C Dieamant; Claudia G Petersen; Ana L Mauri; V Comar; Mariana Mattila; Laura D Vagnini; Adriana Renzi; Bruna Petersen; Andreia Nicoletti; João Batista A Oliveira; Ricardo Lr Baruffi; Jose G Franco Journal: JBRA Assist Reprod Date: 2017-09-01