Jingxi Ma1, Yong Luo. 1. The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China.
Abstract
OBJECTIVE: To explore the mechanism of electroacupuncture (EA) in improving ischemic stroke. METHODS: The Wistar rat model of focal cerebral ischemia-reperfusion was prepared by the thread embolism method. The rats were randomly divided into a normal group, a model group, and an EA group. EA was given at bilateral "Hegu" (LI 4) in rats of the EA group. Expression of the vascular endothelial growth factor (VEGF) mRNA was detected with hybridization in situ, and expressions of the angiogenin-1 (Ang-1) and endostatin proteins were detected with the immunohistochemical method. RESULTS: As compared with the normal group, the expressions of VEGF mRNA, Ang-1 protein and endostatin protein significantly increased in the model group (all P < 0.05); and when compared with the model group, the EA group showed even more significant increase in expressions of the VEGF mRNA and Ang-1 protein (both P < 0.05), but with an obvious decline in the increase of expression of endostatin protein (P < 0.05). CONCLUSIONS: EA can promote angiogenesis in brain of experimental cerebral ischemic rats after reperfusion probably through up-regulating the expression of angiogenesis factors and down-regulating the expression of anti-angiogenesis factors.
OBJECTIVE: To explore the mechanism of electroacupuncture (EA) in improving ischemic stroke. METHODS: The Wistar rat model of focal cerebral ischemia-reperfusion was prepared by the thread embolism method. The rats were randomly divided into a normal group, a model group, and an EA group. EA was given at bilateral "Hegu" (LI 4) in rats of the EA group. Expression of the vascular endothelial growth factor (VEGF) mRNA was detected with hybridization in situ, and expressions of the angiogenin-1 (Ang-1) and endostatin proteins were detected with the immunohistochemical method. RESULTS: As compared with the normal group, the expressions of VEGF mRNA, Ang-1 protein and endostatin protein significantly increased in the model group (all P < 0.05); and when compared with the model group, the EA group showed even more significant increase in expressions of the VEGF mRNA and Ang-1 protein (both P < 0.05), but with an obvious decline in the increase of expression of endostatin protein (P < 0.05). CONCLUSIONS: EA can promote angiogenesis in brain of experimental cerebral ischemicrats after reperfusion probably through up-regulating the expression of angiogenesis factors and down-regulating the expression of anti-angiogenesis factors.
Authors: Reggie H C Lee; Michelle H H Lee; Celeste Y C Wu; Alexandre Couto E Silva; Harlee E Possoit; Tsung-Han Hsieh; Alireza Minagar; Hung Wen Lin Journal: Neural Regen Res Date: 2018-03 Impact factor: 5.135
Authors: Ji Hyun Kim; Kyung Ha Choi; Young Jung Jang; Ha Neui Kim; Sun Sik Bae; Byung Tae Choi; Hwa Kyoung Shin Journal: BMC Complement Altern Med Date: 2013-01-28 Impact factor: 3.659
Authors: Branden A Smeester; Mona Al-Gizawiy; Elaine E O'Brien; Marna E Ericson; Jennifer L Triemstra; Alvin J Beitz Journal: Evid Based Complement Alternat Med Date: 2013-10-21 Impact factor: 2.629