Literature DB >> 19003320

Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: Epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8.

A Ichikawa1, S Y Shim, Y Katakura, K Teruya, S Hashizume, S Shirahata.   

Abstract

We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 were studied by using synthetic linear peptide scanning technology. To determine the locations of B cell epitope, 48 and 95 of decapeptides covering the entire 14-3-3 proteins and CK8, respectively,were synthesized and binding to AE6F4 antibody was examined by ELISA. The AE6F4 antibody was strongly reactive to peptides containing amino acid sequences TLWTSDTQGD in 14-3-3 proteins and INFLRQLYEE in CK8. These results indicate that AE6F4 antibody can recognize the different peptide sequences in 14-3-3 proteins and CK8.

Entities:  

Year:  2001        PMID： 19003320      PMCID： PMC3449674          DOI： 10.1023/A:1014060113186

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  10 in total

1.  In vitro immunization of human lymphocytes with human lung cancer cell line A549.

Authors:  H Kawahara; S Shirahata; H Tachibana; H Murakami
Journal:  Hum Antibodies Hybridomas       Date:  1992-01

2.  Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation.

Authors:  M P Woodward; W W Young; R A Bloodgood
Journal:  J Immunol Methods       Date:  1985-04-08       Impact factor: 2.303

3.  Identification of genes overexpressed in head and neck squamous cell carcinoma using a combination of complementary DNA subtraction and microarray analysis.

Authors:  D B Villaret; T Wang; D Dillon; J Xu; D Sivam; M A Cheever; S G Reed
Journal:  Laryngoscope       Date:  2000-03       Impact factor: 3.325

4.  Elevated expression levels of the 14-3-3 family of proteins in lung cancer tissues.

Authors:  K Nakanishi; S Hashizume; M Kato; T Honjoh; Y Setoguchi; K Yasumoto
Journal:  Hum Antibodies       Date:  1997

5.  Cytokeratin 8 and 19 as antigens recognized by adenocarcinoma-reactive human monoclonal antibody AE6F4.

Authors:  A Ichikawa; H Tachibana; S Kawamoto; M Kamei; T Honjoh; S Hashizume; S Shirahata
Journal:  Hum Antibodies       Date:  1997

6.  Molecular cloning of the 31 kDa cytosolic phospholipase A2, as an antigen recognized by the lung cancer-specific human monoclonal antibody, AE6F4.

Authors:  S Kawamoto; M Shoji; Y Setoguchi; M Kato; S Hashizume; A Ichikawa; K Osada; Y Katakura; H Tachibana; H Murakami
Journal:  Cytotechnology       Date:  1995       Impact factor: 2.058

7.  The 14-3-3 protein as the antigen for lung cancer-associated human monoclonal antibody AE6F4.

Authors:  M Shoji; S Kawamoto; Y Setoguchi; K Mochizuki; T Honjoh; M Kato; S Hashizume; T Hanagiri; T Yoshimatsu; K Nakanishi
Journal:  Hum Antibodies Hybridomas       Date:  1994

8.  Specific reactivity of human monoclonal antibody AE6F4 against cancer cells in tissues and sputa from lung cancer patients.

Authors:  M Shoji; S Kawamoto; S Sato; M Kamei; M Kato; S Hashizume; K Seki; K Yasumoto; A Nagashima; H Nakahashi
Journal:  Hum Antibodies Hybridomas       Date:  1994

9.  Cytokeratin 19 fragments: a new marker for non-small-cell lung cancer.

Authors:  P Stieber; H Bodenmüller; D Banauch; U Hasholzner; A Dessauer; B Ofenloch-Hähnle; D Jaworek; A Fateh-Moghadam
Journal:  Clin Biochem       Date:  1993-08       Impact factor: 3.281

10.  Increased expression of cytokeratins CK8 and CK19 is associated with head and neck carcinogenesis.

Authors:  X C Xu; J S Lee; S M Lippman; J Y Ro; W K Hong; R Lotan
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  1995-12       Impact factor: 4.254
  10 in total

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