Literature DB >> 19003255

Generation of mouse-human hybridomas secreting antibodies against peanut allergen Ara h1.

Hiroshi Shinmoto1, Yasunori Naganawa, Michie Shimmoto, Soheila J Maleki.   

Abstract

Two clones of mouse-human hybridomas, secreting human monoclonal antibodies to a peanut allergen Ara h1, were generated from human peripheral blood lymphocytes transformed with Epstein--Barr virus, followed by cell fusion with mouse myeloma cells. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed antibody-binding sequences of Ara h1 protein.

Entities:  

Year:  2005        PMID: 19003255      PMCID: PMC3449475          DOI: 10.1007/s10616-005-1578-0

Source DB:  PubMed          Journal:  Cytotechnology        ISSN: 0920-9069            Impact factor:   2.058


  7 in total

1.  The Protein Data Bank and structural genomics.

Authors:  John Westbrook; Zukang Feng; Li Chen; Huanwang Yang; Helen M Berman
Journal:  Nucleic Acids Res       Date:  2003-01-01       Impact factor: 16.971

2.  Biochemical and structural analysis of the IgE binding sites on ara h1, an abundant and highly allergenic peanut protein.

Authors:  D S Shin; C M Compadre; S J Maleki; R A Kopper; H Sampson; S K Huang; A W Burks; G A Bannon
Journal:  J Biol Chem       Date:  1998-05-29       Impact factor: 5.157

Review 3.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

Authors:  S F Altschul; T L Madden; A A Schäffer; J Zhang; Z Zhang; W Miller; D J Lipman
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

4.  Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes.

Authors:  C M Counter; F M Botelho; P Wang; C B Harley; S Bacchetti
Journal:  J Virol       Date:  1994-05       Impact factor: 5.103

5.  Mapping and mutational analysis of the IgE-binding epitopes on Ara h 1, a legume vicilin protein and a major allergen in peanut hypersensitivity.

Authors:  A W Burks; D Shin; G Cockrell; J S Stanley; R M Helm; G A Bannon
Journal:  Eur J Biochem       Date:  1997-04-15

Review 6.  Isolation, identification, and characterization of clones encoding antigens responsible for peanut hypersensitivity.

Authors:  A W Burks; G Cockrell; J S Stanley; R M Helm; G A Bannon
Journal:  Int Arch Allergy Immunol       Date:  1995 May-Jun       Impact factor: 2.749

7.  Immunoglobulin expression by human B lymphocytes clonally transformed by Epstein Barr virus.

Authors:  N A Brown; G Miller
Journal:  J Immunol       Date:  1982-01       Impact factor: 5.422

  7 in total
  2 in total

1.  Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells.

Authors:  Yasunori Naganawa; Michie Shimmoto; Soheila J Maleki; Masayuki Takase; Hiroshi Shinmoto
Journal:  Cytotechnology       Date:  2008-04-11       Impact factor: 2.058

2.  Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2.

Authors:  Hiroshi Shinmoto; Yuji Matsuo; Yasunori Naganawa; Shinichi Tomita; Yuko Takano-Ishikawa
Journal:  Cytotechnology       Date:  2009-12-19       Impact factor: 2.058

  2 in total

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