Literature DB >> 19002917

Characterization of rainbow trout cell lines using microsatellite DNA profiling.

G M Perry1, G J McDonald, M M Ferguson, R C Ganassin, N C Bols.   

Abstract

Ten microsatellite loci (Omy27DU,Omy325(A3)UoG, OmyFGT5TUF,OmyFGT14TUF, OmyFGT15TUF,OmyFGT23TUF, Omy77DU,Ssa20.19NUIG, Ots1BML, andOne18ASC) were amplified using the polymerase chain reaction to create genetic profiles for nine cell lines (RTG-2, RTH-149,RTL-W1,RTgill-W1, RTS-11, RTS-34st, RTP-2, RTP-91E and RTP-91F) from rainbow trout(Oncorhynchus mykiss) and one cell line (CHSE-214) from Chinook salmon (O. tschawytscha). A cell line (PHL) from anon-salmonid, the Pacific herring (Clupea harengus pallasi), was included as a control. The ten loci clearly revealed the uniqueness of each cell line, except for two cell lines (RTP-91E andRTP-91F) from the same fish. RTP-91E and RTP-91F were identical at all loci except Ssa20.19NUIG. The most useful locus for demonstrating uniqueness was Ots1BML. The information was used to demonstrate that an uncharacterized rainbow trout cell line (Clone 1A)was in fact CHSE-214, illustrating the usefulness of multiplexed microsatellites for the creation of genetic profiles for salmonid cell lines and for the testing of cell line cross-contamination.

Entities:  

Year:  2001        PMID： 19002917      PMCID： PMC3449788          DOI： 10.1023/A:1020516804173

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  25 in total

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7.  Protein and microsatellite single locus variability in Salmo salar L. (Atlantic salmon).

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Review 1.  Development, characterization, conservation and storage of fish cell lines: a review.

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Review 3.  Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.

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Review 5.  Applications and potential uses of fish gill cell lines: examples with RTgill-W1.

Authors:  L E J Lee; V R Dayeh; K Schirmer; N C Bols
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  5 in total

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